Evaluation of banana blood disease resistant trait and genetic analysis in Thai banana germplasm: a step towards fertile improved diploid development
摘要
This study investigated the resistance of 85 Thai banana germplasm accessions to banana blood disease (BBD), revealing a wide range of resistance levels to Ralstonia syzygii subsp. celebesensis (Rsc) isolates BT4101 and MY4101. Based on Disease Severity Index (DSI) values, the accessions were grouped into five categories: 2 exhibited high resistance (HR), 7 showed resistance (R), 10 were moderately resistant (MR), 43 were susceptible (S), and 23 were highly susceptible (HS). Genetic diversity analysis, utilizing 8,940 SNPs, identified two primary clusters within the germplasm, highlighting both genetic and morphological diversity while pinpointing cultivars with strong breeding potential due to their resistance. The study also explored allele transmission in improved diploid cultivars through crossbreeding, particularly between a fertile wild type and a resistant, low-fertility cultivar. Among the seven F1 progenies evaluated, HB266 displayed significant resistance to BBD, demonstrating effective allele transfer and underscoring its potential for future breeding programs. This research not only enhances our understanding of resistance mechanisms within Thai banana germplasm but also lays a foundation for the development of cultivars capable of withstanding BBD and other diseases, contributing to the sustainability of banana production.