Identification of quinoa B3 gene family and its expression pattern in response to low temperature stress

B3 family is a gene family containing B3 domain in a group of proteins, which plays an important regulatory role in plant growth and development. Quinoa is not only rich in nutrition, but also has important research significance in stress resistance. In this study, a total of 35 quinoa B3 genes were identified by bioinformatics methods and divided into three subfamilies. Through collinearity analysis, it was found that the evolution of quinoa B3 genes was mainly carried out by fragment replication, and most of the quinoa B3 genes were related to auxin signal response. The 2000 bp region of quinoa B3 gene family promoter contains a large number of hormone and stress response cis-acting elements. Then we found that the expression of B3 gene in low temperature tolerant quinoa lines was higher than that in low temperature sensitive quinoa lines under low temperature stress by sequencing transcriptome data and Quantitative Real-time PCR analysis. It can be seen that the quinoa B3 gene is induced to express under low temperature stress, and it is speculated that it plays an important role in coping with low temperature stress. In this study, the main functions of quinoa B3 gene and its role under low temperature stress were explored, which provided theoretical basis for the study of the role of quinoa B3 in quinoa resistance to low temperature stress.