Sequence-related amplified polymorphism (SRAP) markers reveal genetic variation of rice strains obtained by millet DNA injection through coleoptile

Rice represents one of important cereal crops on which half the world’s population depend and it is cultivated on a large scale in Asia. To assist managing rice germplasm, genetic relationship among 48 rice genotypes including MR-4 and MR-11 obtained by coleoptile-mediated millet DNA injection was investigated using sequence-related amplified polymorphism (SRAP) markers. A total of 17 SRAP primer pairs exhibiting reproducible amplification characteristics out of combinations from Me1-Me5, Em1-Em4 were used. Genetic similarity between 48 genotypes revealed by 17 SRAP primer pairs ranged from 0.834 to 0.985. Unweighted pair group method with arithmetic average (UPGMA) tree based on SRAP marker analysis divided 48 rice into two main clusters, that was nearly consistent with classification by growth period. DNA receptor, Sohaechal 16 belonged to second subcluster of Cluster II and MR-4 and MR-11 obtained by introducing millet DNA were assigned to first subcluster of Cluster I. These results suggest that both MR-4 and MR-11 may be considered as distinct germplasm diffferent from DNA receptor Sohaechal 16. Detailed information on genetic relationship of 48 rice genotypes revealed by SRAP markers would contribute to germplasm improvement in the future.