Scholarlink

<p>N6-methyladenosine (m<sup>6</sup>A) is a prevalent modification of eukaryotic mRNAs that plays a crucial role in gene regulation and genome integrity. YT521-B homology (YTH) domain-containing RNA-binding proteins act as essential m6A readers, influencing the fate of m<sup>6</sup>A-modified RNAs through their involvement in RNA splicing, processing, stability, and translation. In plants, <i>YTH</i> genes regulate plant growth and development by modulating these post-transcriptional processes. Despite the significance of barley (<i>Hordeum vulgare</i> L) as a staple crop, the <i>YTH</i> genes in this species remain largely unexplored. We conducted a detailed analysis of the barley genome and identified 14 <i>YTH</i> genes. Phylogenetic classification categorized these genes into 5 distinct groups. These genes are distributed across seven chromosomes, and their predicted protein products are primarily localized within the nucleus. We observed conserved exon structures and domains among the various groups of <i>HvYTHs</i>. Analysis of the promoter region identified several regulatory elements associated with developmental processes, stress responses, and hormone regulation. Protein-protein interaction predictions suggested associations with m<sup>6</sup>A methyltransferase components and stress-responsive factors. Additionally, miRNA target analysis identified potential post-transcriptional regulators of <i>HvYTH</i> genes. Expression profiling using RNA-seq data revealed both tissue-specific and stress-responsive patterns. Several <i>HvYTH</i> genes showing differential expression under cold, heat, and heavy metal stress. qRT-PCR validation confirmed the upregulation of <i>HvYTH8</i> across all stress conditions, while <i>HvYTH5</i>,<i> HvYTH10</i>, and <i>HvYTH12</i> members exhibited stress-specific upregulation or downregulation. These results underscore the functional divergence of <i>HvYTH</i> genes in mediating abiotic stress tolerance, providing potential targets for improving barley resilience.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-wide identification and expression analysis of YTH gene family in barley reveals their potential role under abiotic stresses

  • 期刊论文

  • 发表日期：

    2026年2月12日

查看全文
  • Muhammad Mudasir,
  • Muhammad Shahzad,
  • Ayesha Bibi,
  • Shareef Gul,
  • Liu Qiuyi,
  • Sarmad Frogh Arshad,
  • Hameed Gul,
  • Ali Shahzad

摘要

N6-methyladenosine (m6A) is a prevalent modification of eukaryotic mRNAs that plays a crucial role in gene regulation and genome integrity. YT521-B homology (YTH) domain-containing RNA-binding proteins act as essential m6A readers, influencing the fate of m6A-modified RNAs through their involvement in RNA splicing, processing, stability, and translation. In plants, YTH genes regulate plant growth and development by modulating these post-transcriptional processes. Despite the significance of barley (Hordeum vulgare L) as a staple crop, the YTH genes in this species remain largely unexplored. We conducted a detailed analysis of the barley genome and identified 14 YTH genes. Phylogenetic classification categorized these genes into 5 distinct groups. These genes are distributed across seven chromosomes, and their predicted protein products are primarily localized within the nucleus. We observed conserved exon structures and domains among the various groups of HvYTHs. Analysis of the promoter region identified several regulatory elements associated with developmental processes, stress responses, and hormone regulation. Protein-protein interaction predictions suggested associations with m6A methyltransferase components and stress-responsive factors. Additionally, miRNA target analysis identified potential post-transcriptional regulators of HvYTH genes. Expression profiling using RNA-seq data revealed both tissue-specific and stress-responsive patterns. Several HvYTH genes showing differential expression under cold, heat, and heavy metal stress. qRT-PCR validation confirmed the upregulation of HvYTH8 across all stress conditions, while HvYTH5, HvYTH10, and HvYTH12 members exhibited stress-specific upregulation or downregulation. These results underscore the functional divergence of HvYTH genes in mediating abiotic stress tolerance, providing potential targets for improving barley resilience.