Scholarlink

<p>Anadromous fishes must allocate endogenous reserves to upstream migration and gonadal development, yet tissue-specific lipid organization across female reproductive states remains poorly resolved at the molecular-species level. We applied quantitative UHPLC-QTRAP MS/MS lipidomics to matched gonad, liver, and muscle samples from 18 wild <i>Coilia nasus</i> collected during the 2024 Yangtze River spawning migration. The design included early-developing males (stages II–III, <i>n</i> = 6), early-developing females (stages II–III, <i>n</i> = 6), and maturing females (stages IV–V, <i>n</i> = 6). Overall, 1188 lipid species from 43 subclasses were quantified using an internal-standard-based targeted workflow. Tissue identity was the dominant source of lipidomic variation (PERMANOVA, <i>R</i><sup>2</sup> = 0.333, <i>p</i> = 0.001). The combined reproductive-stage/sex factor explained additional variance (<i>R</i><sup>2</sup> = 0.101, <i>p</i> = 0.001), but this effect was interpreted cautiously because dispersion differed among groups. The ovary showed the clearest FDR-supported lipidomic remodeling. Maturing females had lower gonadal triacylglycerol and higher phosphatidylcholine, lysophosphatidylcholine, lysophosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol, cholesterol, lysophosphatidic acid, phosphatidic acid, and hexosylceramide. Gonadal phosphatidylcholine and phosphatidylethanolamine fractions showed higher weighted mean chain length and unsaturation, and EPA- and DHA-containing phospholipid fractions were selectively enriched, including a significant increase in the DHA-containing phosphatidylcholine fraction. At the lipid-species level, 346 gonadal lipid molecular species met the predefined criteria of FDR &lt; 0.05 and |log<sub>2</sub>FC|&#xa0;≥ 1 in the female gonadal comparison. By contrast, liver and muscle showed broader nominal differences, but no subclass-level comparison remained significant after FDR filtering. These results identify ovarian phospholipid remodeling as the principal tissue-level lipidomic feature associated with female reproductive state in wild migratory <i>C. nasus</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Multi-tissue quantitative lipidomics reveals ovarian phospholipid remodeling across female reproductive states in anadromous Coilia nasus

  • 期刊论文

  • 发表日期：

    2026年7月23日

查看全文
  • Hongyi Guo,
  • Xuguang Zhang,
  • Wenqiao Tang,
  • Kai Liu

摘要

Anadromous fishes must allocate endogenous reserves to upstream migration and gonadal development, yet tissue-specific lipid organization across female reproductive states remains poorly resolved at the molecular-species level. We applied quantitative UHPLC-QTRAP MS/MS lipidomics to matched gonad, liver, and muscle samples from 18 wild Coilia nasus collected during the 2024 Yangtze River spawning migration. The design included early-developing males (stages II–III, n = 6), early-developing females (stages II–III, n = 6), and maturing females (stages IV–V, n = 6). Overall, 1188 lipid species from 43 subclasses were quantified using an internal-standard-based targeted workflow. Tissue identity was the dominant source of lipidomic variation (PERMANOVA, R2 = 0.333, p = 0.001). The combined reproductive-stage/sex factor explained additional variance (R2 = 0.101, p = 0.001), but this effect was interpreted cautiously because dispersion differed among groups. The ovary showed the clearest FDR-supported lipidomic remodeling. Maturing females had lower gonadal triacylglycerol and higher phosphatidylcholine, lysophosphatidylcholine, lysophosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol, cholesterol, lysophosphatidic acid, phosphatidic acid, and hexosylceramide. Gonadal phosphatidylcholine and phosphatidylethanolamine fractions showed higher weighted mean chain length and unsaturation, and EPA- and DHA-containing phospholipid fractions were selectively enriched, including a significant increase in the DHA-containing phosphatidylcholine fraction. At the lipid-species level, 346 gonadal lipid molecular species met the predefined criteria of FDR < 0.05 and |log2FC| ≥ 1 in the female gonadal comparison. By contrast, liver and muscle showed broader nominal differences, but no subclass-level comparison remained significant after FDR filtering. These results identify ovarian phospholipid remodeling as the principal tissue-level lipidomic feature associated with female reproductive state in wild migratory C. nasus.