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<p>This study investigated the effects of dietary 17α-methyltestosterone (MT) on gonadal differentiation and transcriptomic changes associated with partial masculinization in <i>Trachinotus blochii</i>. Larvae (3&#xa0;days post-hatching) were fed a diet containing 100&#xa0;mg/kg MT for 60&#xa0;days. The results showed that MT treatment induced intersex gonadal development in 73% of genetic females, with gonads characterized by the presence of spermatogonia. Hormone assays showed increased MT levels and reduced 17β-estradiol (E2) levels in MT-treated intersex individuals compared with control females. Compared with control females, MT-treated intersex individuals showed increased expression of male-associated genes, including <i>cyp11b1, sf1</i>, and dmrt1, whereas <i>cyp19a1a</i> was reduced. However, other female-associated genes, such as <i>hsd17β1</i> and <i>foxl2</i>, were not completely suppressed. Transcriptome profiling identified significant enrichment of FoxO-related and steroid hormone biosynthesis pathways. Trend analysis suggested that changes in oocyte meiosis-related and steroid hormone biosynthesis-related genes may be associated with MT-induced gonadal remodeling. These findings provide transcriptomic evidence for pathways potentially associated with MT-induced intersex gonadal development and may inform future studies on sex-control strategies in <i>T. blochii</i>, although fertility testing and reproductive validation of these intersex individuals are still required.</p>

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Transcriptomic analysis suggests FoxO and steroid hormone biosynthesis pathways are associated with 17α-methyltestosterone-induced partial masculinization in Trachinotus blochii

  • 期刊论文

  • 发表日期：

    2026年7月14日

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  • Da Zheng,
  • Huapeng Chen,
  • Huan Liang,
  • Jian Luo,
  • Feibiao Song

摘要

This study investigated the effects of dietary 17α-methyltestosterone (MT) on gonadal differentiation and transcriptomic changes associated with partial masculinization in Trachinotus blochii. Larvae (3 days post-hatching) were fed a diet containing 100 mg/kg MT for 60 days. The results showed that MT treatment induced intersex gonadal development in 73% of genetic females, with gonads characterized by the presence of spermatogonia. Hormone assays showed increased MT levels and reduced 17β-estradiol (E2) levels in MT-treated intersex individuals compared with control females. Compared with control females, MT-treated intersex individuals showed increased expression of male-associated genes, including cyp11b1, sf1, and dmrt1, whereas cyp19a1a was reduced. However, other female-associated genes, such as hsd17β1 and foxl2, were not completely suppressed. Transcriptome profiling identified significant enrichment of FoxO-related and steroid hormone biosynthesis pathways. Trend analysis suggested that changes in oocyte meiosis-related and steroid hormone biosynthesis-related genes may be associated with MT-induced gonadal remodeling. These findings provide transcriptomic evidence for pathways potentially associated with MT-induced intersex gonadal development and may inform future studies on sex-control strategies in T. blochii, although fertility testing and reproductive validation of these intersex individuals are still required.