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<p>Heat shock proteins (HSPs) are molecular chaperones that play a crucial role in fish physiology from stress-induced damage by stabilizing proteins and enhancing survival under environmental challenges. This study characterizes the stress-responsive hsp70 (<i>tb-ihsp70</i>) gene in <i>Trachinotus blochii</i>, a prime marine aquaculture species, from an evolutionary perspective and links its expression to salinity and pathogen stress. The <i>tb-ihsp70</i> encodes a 639 amino acid protein with conserved ATPase and substrate-binding domains critical for protein conformation under stress. Phylogenetic analysis grouped it within <i>Carangidae</i>, showing a strong link to <i>Trachinotus ovatus</i>, with marine and freshwater species forming distinct clades, reflecting the environmental role in its phylogeny. Structural modelling revealed a Y-shaped conformation with high-confidence validation scores. STRING analysis identified functional partners, highlighting involvement in broader stress response pathways. The intronless feature and expression profiling classified <i>tb-ihsp70</i> as a constitutively expressed and moderately inducible HSP70. Expression in healthy fish was maximum in gill &gt; posterior kidney &gt; spleen &gt; liver &gt; heart. Salinity stress showed significant upregulation at 3‰ on day two (7.95-fold) and three (5.26-fold), while no significant changes were observed at 42‰ on any day, indicating a stronger and longer <i>tb-ihsp70</i> induction at lower salinity levels. <i>Vibrio harveyi</i> challenge peaked expression at 12&#xa0;h in spleen (73.86-fold), heart (40.88-fold), and posterior kidney (23.10-fold). In conclusion, this first comprehensive characterization underscores the role of <i>tb-ihsp70</i> in biotic and abiotic stress adaptation, supporting its potential as a molecular biomarker for <i>T. blochii</i> health research in mariculture.</p>

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Unravelling the hsp70 gene in snubnose pompano (Trachinotus blochii): insights into stress response mechanisms

  • 期刊论文

  • 发表日期：

    2025年9月23日

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  • Gayathri Suresh,
  • Sumithra Thangalazhy Gopakumar,
  • Krupesha Sharma S. R.,
  • Ambarish Purackattu Gop,
  • Vishnu Suresh Govind,
  • Anusree Velappan Nair,
  • Amritha Jagannivasan,
  • George Joseph Chakkalakkal,
  • Sudarsan Kalappurakal Santhoshkumar,
  • Gopalakrishnan Achamveetil

摘要

Heat shock proteins (HSPs) are molecular chaperones that play a crucial role in fish physiology from stress-induced damage by stabilizing proteins and enhancing survival under environmental challenges. This study characterizes the stress-responsive hsp70 (tb-ihsp70) gene in Trachinotus blochii, a prime marine aquaculture species, from an evolutionary perspective and links its expression to salinity and pathogen stress. The tb-ihsp70 encodes a 639 amino acid protein with conserved ATPase and substrate-binding domains critical for protein conformation under stress. Phylogenetic analysis grouped it within Carangidae, showing a strong link to Trachinotus ovatus, with marine and freshwater species forming distinct clades, reflecting the environmental role in its phylogeny. Structural modelling revealed a Y-shaped conformation with high-confidence validation scores. STRING analysis identified functional partners, highlighting involvement in broader stress response pathways. The intronless feature and expression profiling classified tb-ihsp70 as a constitutively expressed and moderately inducible HSP70. Expression in healthy fish was maximum in gill > posterior kidney > spleen > liver > heart. Salinity stress showed significant upregulation at 3‰ on day two (7.95-fold) and three (5.26-fold), while no significant changes were observed at 42‰ on any day, indicating a stronger and longer tb-ihsp70 induction at lower salinity levels. Vibrio harveyi challenge peaked expression at 12 h in spleen (73.86-fold), heart (40.88-fold), and posterior kidney (23.10-fold). In conclusion, this first comprehensive characterization underscores the role of tb-ihsp70 in biotic and abiotic stress adaptation, supporting its potential as a molecular biomarker for T. blochii health research in mariculture.