Scholarlink

<p>Antioxidant traits in rice are quantitatively inherited and play an important role in improving nutritional quality. However, the genetic basis of antioxidant variation within Thai rice remains unexplored. In this study, we investigated the genetic basis of total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity (AC, measured by ABTS) in a panel of 159 Thai rice cultivars using genome-wide association analysis. A total of 209,594 high-quality SNPs located in promoter and exonic regions were analyzed using a mixed linear model implemented in GEMMA. All three traits exhibited high broad-sense heritability (<i>H</i><sup><i>2</i></sup> = 77.23–86.02%) and strong positive correlations (<i>r</i> = 0.90–0.963, <i>p</i> &lt; 0.01). We identified 158 significant SNPs distributed across multiple chromosomes. Among these, 108 SNPs were associated with at least two antioxidant traits and were consolidated into 38 loci, resulting in eight consensus QTLs. These QTLs collectively explained 10.06–35.81% of phenotypic variation. Within these regions, 14 candidate genes were detected, including previously characterized flavonoid biosynthesis genes—the regulator <i>OsRc</i> and the structural gene <i>OsCHS2</i>—as well as additional MYB, bHLH, and WD-repeat transcription factors. <i>In silico</i> expression profiling using a public database supported that 10 candidates are expressed in seed developmental tissues. Our findings identify promising candidate genes and highlight additional transcriptional factors that may contribute to variation in antioxidant levels in Thai rice germplasm. These results provide valuable genetic resources to facilitate molecular breeding of antioxidant properties in Thai rice.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genome-wide association study of antioxidant compounds and antioxidant activity in a panel of Thai rice cultivars

  • 期刊论文

  • 发表日期：

    2026年6月26日

查看全文
  • Saranyu Thaworn,
  • Phanomsak Tomjai,
  • Teerapong Buaboocha,
  • Supachitra Chadchawan,
  • Monnat Pongpanich,
  • Chanita Paliyavuth,
  • Waraluk Kasettranan

摘要

Antioxidant traits in rice are quantitatively inherited and play an important role in improving nutritional quality. However, the genetic basis of antioxidant variation within Thai rice remains unexplored. In this study, we investigated the genetic basis of total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity (AC, measured by ABTS) in a panel of 159 Thai rice cultivars using genome-wide association analysis. A total of 209,594 high-quality SNPs located in promoter and exonic regions were analyzed using a mixed linear model implemented in GEMMA. All three traits exhibited high broad-sense heritability (H2 = 77.23–86.02%) and strong positive correlations (r = 0.90–0.963, p < 0.01). We identified 158 significant SNPs distributed across multiple chromosomes. Among these, 108 SNPs were associated with at least two antioxidant traits and were consolidated into 38 loci, resulting in eight consensus QTLs. These QTLs collectively explained 10.06–35.81% of phenotypic variation. Within these regions, 14 candidate genes were detected, including previously characterized flavonoid biosynthesis genes—the regulator OsRc and the structural gene OsCHS2—as well as additional MYB, bHLH, and WD-repeat transcription factors. In silico expression profiling using a public database supported that 10 candidates are expressed in seed developmental tissues. Our findings identify promising candidate genes and highlight additional transcriptional factors that may contribute to variation in antioxidant levels in Thai rice germplasm. These results provide valuable genetic resources to facilitate molecular breeding of antioxidant properties in Thai rice.