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<p>Daylilies (<i>Hemerocallis</i> spp.) are widely cultivated for their ornamental, edible, and medicinal values. However, they are under severe threat of rust disease (RD) caused by <i>Puccinia hemerocallidis</i>, which significantly challenges their application. In this study, an F<sub>1</sub> population derived from a cross between the RD-resistant maternal parent ‘Whale Tail (WT)’ and the RD-susceptible paternal parent ‘Carolina French Vanilla (CFV)’ was used to preliminarily identify quantitative trait loci (QTLs) for daylily rust disease resistance based on bulked-segregant analysis (BSA). A total of five candidate QTLs spanning 12.53&#xa0;Mb were identified by both the |Δ(SNP-index)| and Euclidean distance (ED) algorithms, including two QTLs located on chromosome CM030360.1. Additionally, a total of 140 simple sequence repeat (SSR) and 39 insertion-deletion (InDel) markers were used to develop and validate markers associated with rust resistance in daylily. Five SSR markers (SSR-5, SSR-20, SSR-82, XC057, and XC079) and three InDel markers (InDel-8, InDel-18, and InDel-37) were identified as being associated with the target trait. The SSR markers showed validation accuracies ranging from 83 to 100%, with SSR-5 and SSR-20 achieving 100% accuracy. The InDel markers exhibited accuracies of 93% to 100%, with InDel-8 and InDel-37 achieving 100%. The identified QTLs and the molecular markers developed in this study provide potential candidate resources for future marker-assisted selection (MAS) in daylily rust resistance breeding.</p>

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QTL mapping and molecular marker development for rust disease resistance in daylily (Hemerocallis spp.) based on BSA-seq

  • 期刊论文

  • 发表日期：

    2026年5月26日

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  • Jingzhuo Gao,
  • Jinyan Jiang,
  • Qiaochi Tao,
  • Saba Haider,
  • Shijie Zhang,
  • Zhiguo Zhang,
  • Xiang Liu,
  • Di’an Ni,
  • Qiaoping Qin,
  • Min Fan

摘要

Daylilies (Hemerocallis spp.) are widely cultivated for their ornamental, edible, and medicinal values. However, they are under severe threat of rust disease (RD) caused by Puccinia hemerocallidis, which significantly challenges their application. In this study, an F1 population derived from a cross between the RD-resistant maternal parent ‘Whale Tail (WT)’ and the RD-susceptible paternal parent ‘Carolina French Vanilla (CFV)’ was used to preliminarily identify quantitative trait loci (QTLs) for daylily rust disease resistance based on bulked-segregant analysis (BSA). A total of five candidate QTLs spanning 12.53 Mb were identified by both the |Δ(SNP-index)| and Euclidean distance (ED) algorithms, including two QTLs located on chromosome CM030360.1. Additionally, a total of 140 simple sequence repeat (SSR) and 39 insertion-deletion (InDel) markers were used to develop and validate markers associated with rust resistance in daylily. Five SSR markers (SSR-5, SSR-20, SSR-82, XC057, and XC079) and three InDel markers (InDel-8, InDel-18, and InDel-37) were identified as being associated with the target trait. The SSR markers showed validation accuracies ranging from 83 to 100%, with SSR-5 and SSR-20 achieving 100% accuracy. The InDel markers exhibited accuracies of 93% to 100%, with InDel-8 and InDel-37 achieving 100%. The identified QTLs and the molecular markers developed in this study provide potential candidate resources for future marker-assisted selection (MAS) in daylily rust resistance breeding.