Scholarlink

<p>Rice blast, caused by <i>Magnaporthe oryzae</i>, is one of the most destructive diseases of rice (<i>Oryza sativa</i> L.), leading to severe yield losses worldwide. Deployment of resistant cultivars remains the most sustainable and cost-effective management strategy. In this study, 500 temperate rice landraces were evaluated under uniform blast nursery (UBN) conditions, of which 168 accessions exhibited resistance with disease scores of 0–3. The resistant landraces were evaluated for blast resistance following isolate-specific inoculation under controlled conditions<b>,</b> using three virulent <i>M. oryzae</i> isolates (Mo-nwi-32, BLKH-1, and BLL-7) that helped to identify 23 landraces with consistent resistance response. These landraces, along with a susceptible check, were characterized for 17 major blast resistance (R) genes (<i>Pi1</i>, <i>Pi2</i>, <i>Pi40</i>, <i>Pi54</i>, <i>Pi33</i>, <i>Pi9</i>, <i>Pia</i>, <i>Pib</i>, <i>Pigm</i>, <i>Pikh</i>, <i>Pikm</i>, <i>Pish</i>, <i>Pita</i>, <i>Piz</i>, <i>Pi36</i>, <i>Pi38</i>, and <i>Pi42</i>). Four landraces (IC256630, IC4582154, IC462761, IC513430) carried 14 R- alleles each, while three (IC388740, IC56689, IC462799) possessed 13 alleles. In contrast, the susceptible check, <i>Mushk Budji</i>, lacked most of the targeted R genes. Despite the cloning of numerous blast R genes, their durability is often undermined by rapidly evolving pathogen populations. The resistant landraces identified in this study represent valuable genetic resources for novel allele discovery and functional characterization. Genotype-specific molecular screening provides a precise strategy to distinguish resistance donors, which can be used in breeding programs and development of rice cultivars with durable resistance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Allelic variation at major blast resistance loci in Indian rice landraces

  • 期刊论文

  • 发表日期：

    2026年5月18日

查看全文
  • Saba Mir,
  • Nakeeb-Un-Nisa,
  • F. A. Mohiddin,
  • Shabir H. Wani,
  • Gazala H. Khan,
  • Sajad Majeed Zargar,
  • Padma Thakur,
  • Musharib Gull,
  • Mahandiya Iqbal,
  • M. Ashraf Ahangar,
  • Asif B. Shikari,
  • Najeebul Rehman Sofi

摘要

Rice blast, caused by Magnaporthe oryzae, is one of the most destructive diseases of rice (Oryza sativa L.), leading to severe yield losses worldwide. Deployment of resistant cultivars remains the most sustainable and cost-effective management strategy. In this study, 500 temperate rice landraces were evaluated under uniform blast nursery (UBN) conditions, of which 168 accessions exhibited resistance with disease scores of 0–3. The resistant landraces were evaluated for blast resistance following isolate-specific inoculation under controlled conditions, using three virulent M. oryzae isolates (Mo-nwi-32, BLKH-1, and BLL-7) that helped to identify 23 landraces with consistent resistance response. These landraces, along with a susceptible check, were characterized for 17 major blast resistance (R) genes (Pi1, Pi2, Pi40, Pi54, Pi33, Pi9, Pia, Pib, Pigm, Pikh, Pikm, Pish, Pita, Piz, Pi36, Pi38, and Pi42). Four landraces (IC256630, IC4582154, IC462761, IC513430) carried 14 R- alleles each, while three (IC388740, IC56689, IC462799) possessed 13 alleles. In contrast, the susceptible check, Mushk Budji, lacked most of the targeted R genes. Despite the cloning of numerous blast R genes, their durability is often undermined by rapidly evolving pathogen populations. The resistant landraces identified in this study represent valuable genetic resources for novel allele discovery and functional characterization. Genotype-specific molecular screening provides a precise strategy to distinguish resistance donors, which can be used in breeding programs and development of rice cultivars with durable resistance.