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<p>Low temperature stress affects plant development, and Late Embryogenesis Abundant (LEA) proteins can improve the cold resistance of plants. Chrysanthemum (<i>Chrysanthemum morifolium</i>) is a Chinese traditional flower and one of the four major cut flowers in the world. However, the function of LEA genes in Chrysanthemum remains unclear. In this study, the chrysanthemum LEA gene <i>LEA2-2</i> was identified and subjected to sequence analysis using bioinformatics. The tissue specificity and cold stress expression patterns of <i>CmLEA2-2</i> were analyzed. Subsequently, heterologous transformation of <i>CmLEA2-2</i> was performed. The cloned <i>CmLEA2-2</i> measures 459&#xa0;bp, encoding 152 amino acids, with a molecular weight of 17.27&#xa0;kDa. Tissue-specific expression showed higher expression of <i>CmLEA2-2</i> in leaves and stems, and it was initially up-regulated and then down-regulated under cold stress. Furthermore, heterologous expression of <i>CmLEA2-2</i> in <i>Escherichia coli</i> and yeast significantly improved their survival rates under cold stress. In conclusion, our results suggest that <i>CmLEA2-2</i> enhanced the cold tolerance of<i> E. coli</i> and yeast. This research advances the understanding of LEA gene function and provides a foundation for breeding cold-resistant chrysanthemum varieties.</p>

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Heterologous expression of chrysanthemum morifolium late embryogenesis abundant protein gene 2–2 (CmLEA2-2) in E. coli and yeast resistant to low temperature stress

  • 期刊论文

  • 发表日期：

    2026年4月9日

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  • Liping Ren,
  • Tianhao Hu,
  • Dandan Yin,
  • Wenyang Wan,
  • Xianhui Deng,
  • Miao Li,
  • Xiaohan Cao

摘要

Low temperature stress affects plant development, and Late Embryogenesis Abundant (LEA) proteins can improve the cold resistance of plants. Chrysanthemum (Chrysanthemum morifolium) is a Chinese traditional flower and one of the four major cut flowers in the world. However, the function of LEA genes in Chrysanthemum remains unclear. In this study, the chrysanthemum LEA gene LEA2-2 was identified and subjected to sequence analysis using bioinformatics. The tissue specificity and cold stress expression patterns of CmLEA2-2 were analyzed. Subsequently, heterologous transformation of CmLEA2-2 was performed. The cloned CmLEA2-2 measures 459 bp, encoding 152 amino acids, with a molecular weight of 17.27 kDa. Tissue-specific expression showed higher expression of CmLEA2-2 in leaves and stems, and it was initially up-regulated and then down-regulated under cold stress. Furthermore, heterologous expression of CmLEA2-2 in Escherichia coli and yeast significantly improved their survival rates under cold stress. In conclusion, our results suggest that CmLEA2-2 enhanced the cold tolerance of E. coli and yeast. This research advances the understanding of LEA gene function and provides a foundation for breeding cold-resistant chrysanthemum varieties.