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<p>Bacteria fruit blotch (BFB), a seed-borne bacterial disease caused by <i>Acidovorax citrulli</i> (<i>A. citrulli</i>), has led devastating losses on cucurbit industry, particularly in watermelon and melon production. In this study, two melon accessions, resistant line 1185 and susceptible line 1232, were crossed, and F<sub>2</sub> population (88 and 225 individuals) was evaluated across two seasons (F<sub>2_Spring</sub> and F<sub>2_Autumn</sub>). Genetic analysis indicated that BFB resistance in melons exhibit the genetic characteristics of quantitative trait. Bulked segregant analysis sequencing (BSA‑seq) analysis, conducted using the index‑slid method and the Euclidean distance (ED) algorithm, identified six and five resistance‑associated chromosomal intervals located on chromosomes 8, 10 and 12, respectively. Within these intervals, <i>MELO3C008984</i> and <i>MELO3C035750</i> harboured nonsynonymous variants in their exons between two parental lines. Quantitative trait locus (QTL) mapping identified two QTLs <i>qBFB-10.1</i> (1.89&#xa0;Mb) and <i>qBFB-10.2</i> (4.87&#xa0;Mb) on chromosome 10 explaining between 4.57% and 14.75% of the phenotypic variation in BFB resistance within F<sub>2_Spring</sub> and F<sub>2_Autumn</sub> populations. Within two QTLs, <i>MELO3C012364</i>, <i>MELO3C012301</i> and <i>MELO3C012204</i> harboured nonsynonymous variants in their exons between two parental lines. Among these genes, the relative expression levels of <i>MELO3C012364</i> (annotated as cytochrome P450 77A3-like) and <i>MELO3C012204</i> (annotated as putative membrane lipoprotein) differed significantly between the parental lines at 36&#xa0;h post inoculation. The functions of these genes will be further verified in our future study. These findings establish a basis for the functional analysis of genes associated with melon BFB resistance and contribute to elucidating the resistance mechanisms in melon against BFB.</p>

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Analysis of genetic loci associated with resistance to bacteria fruit blotch in melon (Cucumis melo L.)

  • 期刊论文

  • 发表日期：

    2026年1月23日

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  • Sa Rina,
  • Jin Yazhong,
  • Xu Chunmei,
  • Zhang Zhixu,
  • Ren Borui,
  • Liu Linshuai,
  • Guo Yongxia,
  • Sheng Yunyan

摘要

Bacteria fruit blotch (BFB), a seed-borne bacterial disease caused by Acidovorax citrulli (A. citrulli), has led devastating losses on cucurbit industry, particularly in watermelon and melon production. In this study, two melon accessions, resistant line 1185 and susceptible line 1232, were crossed, and F2 population (88 and 225 individuals) was evaluated across two seasons (F2_Spring and F2_Autumn). Genetic analysis indicated that BFB resistance in melons exhibit the genetic characteristics of quantitative trait. Bulked segregant analysis sequencing (BSA‑seq) analysis, conducted using the index‑slid method and the Euclidean distance (ED) algorithm, identified six and five resistance‑associated chromosomal intervals located on chromosomes 8, 10 and 12, respectively. Within these intervals, MELO3C008984 and MELO3C035750 harboured nonsynonymous variants in their exons between two parental lines. Quantitative trait locus (QTL) mapping identified two QTLs qBFB-10.1 (1.89 Mb) and qBFB-10.2 (4.87 Mb) on chromosome 10 explaining between 4.57% and 14.75% of the phenotypic variation in BFB resistance within F2_Spring and F2_Autumn populations. Within two QTLs, MELO3C012364, MELO3C012301 and MELO3C012204 harboured nonsynonymous variants in their exons between two parental lines. Among these genes, the relative expression levels of MELO3C012364 (annotated as cytochrome P450 77A3-like) and MELO3C012204 (annotated as putative membrane lipoprotein) differed significantly between the parental lines at 36 h post inoculation. The functions of these genes will be further verified in our future study. These findings establish a basis for the functional analysis of genes associated with melon BFB resistance and contribute to elucidating the resistance mechanisms in melon against BFB.