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<p>Cytoplasmic malate dehydrogenase (OsMDH) regulates the conversion of malic acid and oxaloacetate in rice and is a key enzyme in sugar metabolism. However, it is still unclear whether <i>OsMDH</i>, which is a constitutive expression gene, affects the quality traits of rice. In this study, we screened 92 regulatory that factors may interact with <i>OsAAP6</i> using yeast single hybridization tests. We than validated the interaction between OsMDH and <i>OsAAP6</i> with local surface plasmon resonance detection tests and point-to-point experiments in yeast. The grain protein content (GPC), amylose content, and chalkiness rate and degree of <i>Osmdh</i> homozygous mutant seeds significantly increased, while the starch content, gel consistency, head milled rice rate (HMRR), and free fatty acid content significantly decreased. Also, the <i>Osmdh</i> mutant seeds had shorter and thicker grains, more protein bodies in the endosperm, and significantly altered corresponding grain qualities. The reverse was true for the starch content, gel consistency, HMRR, free fatty acid content, amylose content, chalkiness rate and degree of <i>OsMDH</i>-overexpressing transgene-positive grains. Transcriptome analysis of <i>Osmdh</i> mutants and <i>OsMDH</i>-overexpressing transgenic materials, and transcript based gene expression depicted that the genes related to the metabolism of major storage substances, revealed that the differentially expressed genes were mainly involved in biological processes such as growth, development and various abiotic stress. Also, the expression levels of the main metabolism-related genes of the storage substance in the <i>Osmdh</i> mutants and the <i>OsMDH-</i>overexpressing transgenic endosperm were consistent with the results of the contents detection. Therefore, <i>OsMDH</i> has a significant impact on the main storage substances of rice grain and the corresponding quality indicators, providing an important genetic resource for the future use in molecular design of high-quality rice breeding.</p>

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OsMDH regulates the accumulation of major storage substances in grains and affects various quality traits of rice

  • 期刊论文

  • 发表日期：

    2025年12月6日

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  • Bo Peng,
  • Xiaoyu Sun,
  • Yan Liu,
  • Qiang Zhao,
  • Yanfang Sun,
  • Jing Peng,
  • Zhiguo Zhang,
  • Jing Qiu,
  • Xiayu Tian,
  • Yaqin Huang,
  • Wei Zhou,
  • Jinhui Zhao,
  • Quanxiu Wang

摘要

Cytoplasmic malate dehydrogenase (OsMDH) regulates the conversion of malic acid and oxaloacetate in rice and is a key enzyme in sugar metabolism. However, it is still unclear whether OsMDH, which is a constitutive expression gene, affects the quality traits of rice. In this study, we screened 92 regulatory that factors may interact with OsAAP6 using yeast single hybridization tests. We than validated the interaction between OsMDH and OsAAP6 with local surface plasmon resonance detection tests and point-to-point experiments in yeast. The grain protein content (GPC), amylose content, and chalkiness rate and degree of Osmdh homozygous mutant seeds significantly increased, while the starch content, gel consistency, head milled rice rate (HMRR), and free fatty acid content significantly decreased. Also, the Osmdh mutant seeds had shorter and thicker grains, more protein bodies in the endosperm, and significantly altered corresponding grain qualities. The reverse was true for the starch content, gel consistency, HMRR, free fatty acid content, amylose content, chalkiness rate and degree of OsMDH-overexpressing transgene-positive grains. Transcriptome analysis of Osmdh mutants and OsMDH-overexpressing transgenic materials, and transcript based gene expression depicted that the genes related to the metabolism of major storage substances, revealed that the differentially expressed genes were mainly involved in biological processes such as growth, development and various abiotic stress. Also, the expression levels of the main metabolism-related genes of the storage substance in the Osmdh mutants and the OsMDH-overexpressing transgenic endosperm were consistent with the results of the contents detection. Therefore, OsMDH has a significant impact on the main storage substances of rice grain and the corresponding quality indicators, providing an important genetic resource for the future use in molecular design of high-quality rice breeding.