<p>Soybean (<i>Glycine max</i>), a member of the Fabaceae family, is a globally important source of protein and oil, making its disease resistance a critical agricultural trait. This review systematically summarizes recent progress on the synergistic defense mechanisms between soybean disease resistance genes and stomatal regulation. Stomata, as major entry points for pathogens, serve as dynamic immune hubs integrating hormonal and environmental signals. Resistance genes, particularly NLR genes activate immune responses by specifically recognizing pathogenic effectors, while non-canonical resistance genes participate in defense through regulating oxidative defense and ion homeostasis. Key stomatal regulatory components, including SLAC1, OST1, GHR1 and MAPK cascades mediate rapid stomatal closure in response to pathogen or PAMP triggered signals, often in-coordination with plant hormone pathways such as ABA, SA, and JA. Multi-omics studies have revealed co-expression and interaction networks between resistance genes and stomatal regulation genes, providing a theoretical framework for molecular breeding. Future research should focus on manipulating stomatal regulatory networks via gene editing and synthetic biology approaches which hold great potential for breeding disease-resistant soybean cultivars.</p>