<p>Knowing the genetic diversity of genotypes in breeding programs is essential for understanding the genetic variability among materials, guiding decision-making, and facilitating the pursuit of heterosis. Multivariate techniques employed in this research area facilitate the division of heterotic groups and the identification of experimental genotypes resembling market leaders, thereby indicating their potential for commercialization. This study aimed to assess the high yield, fruit quality and genetic diversity of experimental Tetsukabuto pumpkin hybrids. The experiment, conducted at the Federal University of Lavras (UFLA), evaluated 21 interspecific experimental hybrids (HE) alongside four commercially available controls (Nara, Takayama, Kanda, and Jabras). A randomized block design with three replicates and six plants per plot was implemented. Fruit productivity (all and commercial), fruit characteristics (flesh and rind thickness, internal cavity diameter, fruit height and diameter), fruit quality (soluble solids content, color parameters, rind texture, and flesh moisture), and visual appearance (fruit shape, fruit size, fruit uniformity, water-soaked spot presence, and rind and flesh color) were assessed. Best linear unbiased prediction values were estimated, followed by cluster analysis using the UPGMA method for traits exhibiting significant genotypic effects. Principal component analysis was also performed. The results revealed substantial genetic divergence among the hybrids, forming four distinct clusters. A group that encompassed HE-01, HE-12, HE-14, HE-22, and Takayama exhibited superior performance in both all and commercial fruit yield, along with greater fruit height and a desirable fruit shape. This study pinpoints average commercial fruit weight, fruit size, minimal water-soaked spot occurrence, optimal rind texture, and rind color as crucial traits for selecting promising lines in future breeding programs.</p>