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<p><i>Primula forbesii</i>, a dimorphic species with a short growth cycle and high ornamental value, is an important model for studying distyly. However, the absence of a reliable genetic transformation system has limited molecular research in this species. In this study, we monitored pollen germination and pollen tube growth within the pistil to determine the optimal timing for transformation. We further examined the effects of transformation buffer composition, Agrobacterium cell density, and infection method on transformation efficiency. Based on these analyses, we established a stable pollen tube–mediated genetic transformation protocol for <i>P. forbesii</i>. The transformation efficiency reached 5.33%, as confirmed by resistance screening and qPCR detection of exogenous genes. Our findings provide an efficient and robust transformation method that will facilitate molecular studies and breeding applications in <i>P. forbesii</i>.</p>

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Establishment of pollen tube-mediated genetic transformation technique of Primula forbesii

  • 期刊论文

  • 发表日期：

    2025年10月13日

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  • Sitong Fu,
  • Weijia Si,
  • Ying Liu,
  • Jia Wang,
  • Tangren Cheng,
  • Qixiang Zhang,
  • Huitang Pan

摘要

Primula forbesii, a dimorphic species with a short growth cycle and high ornamental value, is an important model for studying distyly. However, the absence of a reliable genetic transformation system has limited molecular research in this species. In this study, we monitored pollen germination and pollen tube growth within the pistil to determine the optimal timing for transformation. We further examined the effects of transformation buffer composition, Agrobacterium cell density, and infection method on transformation efficiency. Based on these analyses, we established a stable pollen tube–mediated genetic transformation protocol for P. forbesii. The transformation efficiency reached 5.33%, as confirmed by resistance screening and qPCR detection of exogenous genes. Our findings provide an efficient and robust transformation method that will facilitate molecular studies and breeding applications in P. forbesii.