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<p>Distant hybridization is a primary method for generating new variations, expanding the genetic foundation of species, and creating new types or varieties. As significant ornamental plants in the Magnoliaceae family, <i>Magnolia liliiflora</i> Desr. and <i>Michelia figo</i> (Lour.) Spreng. exhibit notable differences in floral morphology and color, making them excellent parental materials for hybrid breeding. This study conducted distant hybridization experiments using <i>M. liliiflora</i> as the maternal parent and <i>M. figo </i>as the paternal parent. Observations were made on flowering dynamics, stigma receptivity, pollen viability, fertilization processes, and fruit development status, along with monitoring the growth traits of hybrid seedlings. The results indicated a significant flowering period mismatch between <i>M. liliiflora </i>and <i>M. figo</i>. The paternal parent,&#xa0;<i>M. figo</i>, exhibited the highest pollen viability during the semi-open flower stage.&#xa0;After being stored at − 70&#xa0;°C for nearly one year, its pollen vitality ranked at 51.73 ± 3.64%. The slightly open stage&#xa0;of flowers in <i>M. liliiflora</i> was the optimal time for cross-pollination. <i>M. figo</i> pollen could successfully germinate on the stigmas of <i>M. liliiflora,</i> and pollen tubes elongated normally, completing fertilization within 80&#xa0;h. The ovary enlargement rates in <i>M. liliiflora</i> were 64.13 ± 5.23% and 78.42 ± 4.16%, with fruit set rates of 43.33% and 53.33% in two consecutive years of hybridization experiments, respectively. The germination rate of hybrid seeds from the first year was 39.34%, and the seedling emergence rate was 19.67%. Despite the mismatch in flowering times, effective pollination could still be achieved during the receptive period of <i>M. liliflora</i> stigma with the assistance of low temperature storage technology. Although the seed germination and seedling emergence rates of the hybrid offspring were relatively low, hybrid seedlings were successfully cultivated. These findings provide valuable data for the creation of new plant germplasm in Magnoliaceae.</p>

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The optimal workflow for interspecific hybridization between Magnolia liliiflora Desr. and Michelia figo (Lour.) Spreng., two medicinal and aromatic species endemic to China

  • 期刊论文

  • 发表日期：

    2025年8月4日

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  • Sitong Liu,
  • Liyong Sun,
  • Yao Chen,
  • Lina Xu,
  • Zengfang Yin

摘要

Distant hybridization is a primary method for generating new variations, expanding the genetic foundation of species, and creating new types or varieties. As significant ornamental plants in the Magnoliaceae family, Magnolia liliiflora Desr. and Michelia figo (Lour.) Spreng. exhibit notable differences in floral morphology and color, making them excellent parental materials for hybrid breeding. This study conducted distant hybridization experiments using M. liliiflora as the maternal parent and M. figo as the paternal parent. Observations were made on flowering dynamics, stigma receptivity, pollen viability, fertilization processes, and fruit development status, along with monitoring the growth traits of hybrid seedlings. The results indicated a significant flowering period mismatch between M. liliiflora and M. figo. The paternal parent, M. figo, exhibited the highest pollen viability during the semi-open flower stage. After being stored at − 70 °C for nearly one year, its pollen vitality ranked at 51.73 ± 3.64%. The slightly open stage of flowers in M. liliiflora was the optimal time for cross-pollination. M. figo pollen could successfully germinate on the stigmas of M. liliiflora, and pollen tubes elongated normally, completing fertilization within 80 h. The ovary enlargement rates in M. liliiflora were 64.13 ± 5.23% and 78.42 ± 4.16%, with fruit set rates of 43.33% and 53.33% in two consecutive years of hybridization experiments, respectively. The germination rate of hybrid seeds from the first year was 39.34%, and the seedling emergence rate was 19.67%. Despite the mismatch in flowering times, effective pollination could still be achieved during the receptive period of M. liliflora stigma with the assistance of low temperature storage technology. Although the seed germination and seedling emergence rates of the hybrid offspring were relatively low, hybrid seedlings were successfully cultivated. These findings provide valuable data for the creation of new plant germplasm in Magnoliaceae.