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<p>Fusarium wilt caused by seed borne fungus (<i>Fusarium oxysporum</i> (Schlechtend: Fr.) f. sp. <i>ciceris</i> (Padwick) Matuo &amp; K. Sato), is one of the most destructive diseases of chickpea that can lead to complete yield loss under favorable conditions. Although traditional breeding efforts have contributed to reducing the effect of wilt disease, introduction of effective resistance into users-preferred varieties and against geographically disperse pathogens remains elusive. In this study, 108 F<sub>2</sub> progeny were obtained by crossing the wilt susceptible genotype JG62 with the resistant variety Dera. Disease phenotyping in a Fusarium sick plot revealed the expected resistant and susceptible phenotypes of parental controls, while F<sub>2</sub> hybrids segregated for the parental phenotypes wilt responses: fully susceptible and highly resistant. Genotyping-by-sequencing (GBS) identified 1,659 single nucleotide polymorphisms (SNPs) that distinguish the two parental lines and that segregate with minor allele frequencies &gt; 0.25. After quality filtering and removal of outliers, 836 high quality SNP markers were assigned to six genetic linkage groups (LGs) with a total map size of 274.9&#xa0;cM and 3.12&#xa0;cM average distance between mapped markers. A single major effect QTL, explaining 55.28% of the observed phenotypic variation, was identified on chromosome 4 at 44.29&#xa0;cM with a logarithm of odds (LOD) score of 13.8. This novel QTL and associated genetic markers offer molecular tools for breeding wilt resistant against Fusarium strains. Therefore, fine mapping with a larger population and high-density markers is recommended to precisely delineate the candidate gene responsible for wilt resistance in Chickpea.</p>

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Marker trait association analysis identified a novel quantitative trait loci (QTL) for Fusarium wilt resistance in chickpea (Cicer arietinum L.)

  • 期刊论文

  • 发表日期：

    2025年7月31日

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  • Dagnachew Bekele,
  • Kassahun Tesfaye,
  • Asnake Fikre,
  • Noelia C. Garcia,
  • Peter L. Chang,
  • Douglas R. Cook

摘要

Fusarium wilt caused by seed borne fungus (Fusarium oxysporum (Schlechtend: Fr.) f. sp. ciceris (Padwick) Matuo & K. Sato), is one of the most destructive diseases of chickpea that can lead to complete yield loss under favorable conditions. Although traditional breeding efforts have contributed to reducing the effect of wilt disease, introduction of effective resistance into users-preferred varieties and against geographically disperse pathogens remains elusive. In this study, 108 F2 progeny were obtained by crossing the wilt susceptible genotype JG62 with the resistant variety Dera. Disease phenotyping in a Fusarium sick plot revealed the expected resistant and susceptible phenotypes of parental controls, while F2 hybrids segregated for the parental phenotypes wilt responses: fully susceptible and highly resistant. Genotyping-by-sequencing (GBS) identified 1,659 single nucleotide polymorphisms (SNPs) that distinguish the two parental lines and that segregate with minor allele frequencies > 0.25. After quality filtering and removal of outliers, 836 high quality SNP markers were assigned to six genetic linkage groups (LGs) with a total map size of 274.9 cM and 3.12 cM average distance between mapped markers. A single major effect QTL, explaining 55.28% of the observed phenotypic variation, was identified on chromosome 4 at 44.29 cM with a logarithm of odds (LOD) score of 13.8. This novel QTL and associated genetic markers offer molecular tools for breeding wilt resistant against Fusarium strains. Therefore, fine mapping with a larger population and high-density markers is recommended to precisely delineate the candidate gene responsible for wilt resistance in Chickpea.