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<p>The seedling stage is critically important for the development of rice plants, and rapid shoot growth has a major effect on plant morphology and yield potential. In this study, we identified a major and stable quantitative trait loci (QTL), <i>qSL10</i>, on chromosome 10, that controls shoot growth during the seedling stage using a high-resolution genetic map of recombinant inbred lines (RILs). Through differential gene expression and sequence analysis, we identified the candidate gene <i>OsSL10</i> within the <i>qSL10</i> region. <i>OsSL10</i> was differentially expressed in the shoots of rice varieties with varying shoot growth rates. Functional validation using CRISPR/Cas9-mediated knockout and overexpression lines confirmed that <i>OsSL10</i> regulates shoot length; knockout lines showed reduced shoot growth, and the growth of overexpression lines was enhanced. These findings highlight that <i>OsSL10</i> is a key regulator of seedling shoot development; <i>OsSL10</i> provides a promising target for improving seedling vigor in rice. Furthermore, the identification of new QTLs for seedling growth provides valuable insights into the genetic mechanisms governing early rice development, as well as opportunities for the breeding of high-yielding rice varieties.</p>

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OsSL10, a candidate gene for a major and stable QTL, positively regulates the shoot growth of rice seedlings

  • 期刊论文

  • 发表日期：

    2025年5月13日

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  • Qi Zhong,
  • Mvuyeni Nyasulu,
  • Guoliang Cao,
  • Jun Yang,
  • Liang Chen,
  • Xiaopeng He,
  • Xiaorong Chen,
  • Junru Fu,
  • Dahu Zhou,
  • Jie Xu,
  • Linjuan Ouyang,
  • Haohua He,
  • Jianmin Bian

摘要

The seedling stage is critically important for the development of rice plants, and rapid shoot growth has a major effect on plant morphology and yield potential. In this study, we identified a major and stable quantitative trait loci (QTL), qSL10, on chromosome 10, that controls shoot growth during the seedling stage using a high-resolution genetic map of recombinant inbred lines (RILs). Through differential gene expression and sequence analysis, we identified the candidate gene OsSL10 within the qSL10 region. OsSL10 was differentially expressed in the shoots of rice varieties with varying shoot growth rates. Functional validation using CRISPR/Cas9-mediated knockout and overexpression lines confirmed that OsSL10 regulates shoot length; knockout lines showed reduced shoot growth, and the growth of overexpression lines was enhanced. These findings highlight that OsSL10 is a key regulator of seedling shoot development; OsSL10 provides a promising target for improving seedling vigor in rice. Furthermore, the identification of new QTLs for seedling growth provides valuable insights into the genetic mechanisms governing early rice development, as well as opportunities for the breeding of high-yielding rice varieties.