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<p>Greenbug (<i>Schizaphis graminum</i>) is a key pest that often causes significant damage to cereal crops such as barley, sorghum and wheat. Tremendous efforts of linkage mapping have led to the identification of quantitative trait locus (QTLs) associated with host resistance to greenbug; however, resistance gene(s) or precise location of the chromosome responsible for aphid resistance remains uncharacterized. In this study, we built upon previous research to identify resistance gene(s) in sorghum using a fine mapping approach. A population of 96 F2:7 recombinant inbred lines (RILs) was developed through a cross between BTx623 and the resistant line PI 607900. Critical RILs and newly developed molecular markers were then used for fine mapping, leading to the identification of a major resistance QTL, designated <i>SgR1</i> (<i>Schizaphis graminum Resistance 1</i>). Evidently, the <i>SgR1</i> was narrowed down from the previously known 508&#xa0;kb region on chromosome 9 to an 82.7-kb interval flanked by DNA markers GSR345 and GSR348. Further analysis revealed ten predicted genes in this region, of which four genes encode nucleotide-binding site leucine-rich repeat (NBS-LRR) proteins, indicating that the NBS-LRR genes are the candidates for greenbug resistance. Furthermore, genomic comparison of the <i>SgR1</i> region within the&#xa0;grass genus suggested the four predicted <i>NBS-LRRs</i>&#xa0;that are sorghum-specific occurred after the divergence of sorghum and maize. DNA marker GSR345 proved to be a reliable diagnostic tool for greenbug resistance among twenty selected sorghum accessions. Our study not only offers the opportunity for positional-cloning of the <i>SgR1,</i> but will also facilitate marker-assisted breeding for aphid resistance in sorghum.</p>

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Fine mapping of SgR1, a major gene conferring resistance to greenbug biotype I aphid in sorghum

  • 期刊论文

  • 发表日期：

    2025年3月17日

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  • Hengyou Zhang,
  • Yinghua Huang

摘要

Greenbug (Schizaphis graminum) is a key pest that often causes significant damage to cereal crops such as barley, sorghum and wheat. Tremendous efforts of linkage mapping have led to the identification of quantitative trait locus (QTLs) associated with host resistance to greenbug; however, resistance gene(s) or precise location of the chromosome responsible for aphid resistance remains uncharacterized. In this study, we built upon previous research to identify resistance gene(s) in sorghum using a fine mapping approach. A population of 96 F2:7 recombinant inbred lines (RILs) was developed through a cross between BTx623 and the resistant line PI 607900. Critical RILs and newly developed molecular markers were then used for fine mapping, leading to the identification of a major resistance QTL, designated SgR1 (Schizaphis graminum Resistance 1). Evidently, the SgR1 was narrowed down from the previously known 508 kb region on chromosome 9 to an 82.7-kb interval flanked by DNA markers GSR345 and GSR348. Further analysis revealed ten predicted genes in this region, of which four genes encode nucleotide-binding site leucine-rich repeat (NBS-LRR) proteins, indicating that the NBS-LRR genes are the candidates for greenbug resistance. Furthermore, genomic comparison of the SgR1 region within the grass genus suggested the four predicted NBS-LRRs that are sorghum-specific occurred after the divergence of sorghum and maize. DNA marker GSR345 proved to be a reliable diagnostic tool for greenbug resistance among twenty selected sorghum accessions. Our study not only offers the opportunity for positional-cloning of the SgR1, but will also facilitate marker-assisted breeding for aphid resistance in sorghum.