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<p><i>Anoectochilus roxburghii</i>, as a horticultural plant, is renowned for its distinctive characteristics and widespread cultivation across various Asian regions. The beautiful golden veins make it of great ornamental value. At the same time, it is often used in medicine and food. Due to the immature development of the embryos and seed coat cells, the seeds of <i>A. roxburghii</i> were aborted. Only under the condition of symbiosis with fungi, the germination process of seeds can be promoted. Coupled with the dependence of cross-pollination on natural factors such as wind and insects, the reproduction of <i>A.roxburghii</i> is more complicated and difficult. In this study, we found that exogenous spermidine (Spd) treatment up-regulates the expression of <i>ArWRKY6</i>. Tissue-specific expression analysis showed that <i>ArWRKY6</i> expressed most at the early stage of flower buds period and gradually decreased with the opening of flowers. <i>ArWRKY6</i> overexpression plant demonstrated that it could promote flowering, and positively affected pistil and seed development. Our results provide a foundation for further analysis of the function of WRKY transcription factors in reproductive development of <i>A. roxburghii</i>, and provided a theoretical basis for hybridization breeding.</p>

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Cloning and functional analyzes of Anoectochilus roxburghii WRKY6 which response to spermidine

  • 期刊论文

  • 发表日期：

    2025年3月7日

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  • Yuchan Li,
  • Yulin Ju,
  • Ying Zheng,
  • Jinjing Shi,
  • Liyang Su,
  • Yuqiu Huang,
  • Bingcong Xing,
  • Qingsong Shao,
  • Chenfei Lu

摘要

Anoectochilus roxburghii, as a horticultural plant, is renowned for its distinctive characteristics and widespread cultivation across various Asian regions. The beautiful golden veins make it of great ornamental value. At the same time, it is often used in medicine and food. Due to the immature development of the embryos and seed coat cells, the seeds of A. roxburghii were aborted. Only under the condition of symbiosis with fungi, the germination process of seeds can be promoted. Coupled with the dependence of cross-pollination on natural factors such as wind and insects, the reproduction of A.roxburghii is more complicated and difficult. In this study, we found that exogenous spermidine (Spd) treatment up-regulates the expression of ArWRKY6. Tissue-specific expression analysis showed that ArWRKY6 expressed most at the early stage of flower buds period and gradually decreased with the opening of flowers. ArWRKY6 overexpression plant demonstrated that it could promote flowering, and positively affected pistil and seed development. Our results provide a foundation for further analysis of the function of WRKY transcription factors in reproductive development of A. roxburghii, and provided a theoretical basis for hybridization breeding.