<p>For generations, homegardens have been a source of traditional medicine for the meitei ethnic group of Manipur, northeast India in Indo Burma hotspot. However, the concept of home gardening is disappearing from the localities in the urban and semi-urban parts of the Imphal valley. The changing scenario thereby endangering the existence of the custom and the knowledge associated with the homegarden plants. Therefore, an ethnobotanical homegarden survey was carried out in the Sangaithel (N = 75) region of the valley to highlight the significance of the agricultural spaces in the conservation of different medicinal plants during 2020—2022. In total, 122 ethnomedicinal plants from 103 genera and 54 families were documented and identified. The medicinal plants were used for the treatment of 53 different ailments grouped into 14 categories. The maximum use reports was found for gastro-intestinal or digestive disease category with 48 species used to treat dysentry, indigestion, gastric problem, stomach ulcer, diarrhoea, liver ailment, jaundice, stomach gastritis, tonsilitis, constipation etc. A total of 70 species were edible medicinal plants commonly consumed in the households as traditional delicacies such as chamfut, iromba, kangsu, kanghou, utti, morok metpa etc. Additionally, one critically endangered medicinal plant was protected by the families in their homegardens. The results of the research highlight the significant role of meitei homegardens in the conservation of the medicinal plants used by the ethnic group for generations. Furthermore, it is a necessary requirement today that the concept of home-gardening is kept alive and homegardens as such are protected.</p>