<p>The article underscores the importance of women’s empowerment in achieving SDG-5, “Achieve gender equality and empower all women and girls,” while promoting sustainable tourism within biosphere reserves. Gender equality and women’s empowerment are essential for advancing sustainable development, specifically within UNESCO Biosphere Reserves, which aim to integrate conservation and sustainable resource use. This study is motivated by the limited research on women’s empowerment in biosphere reserves, specifically its role in sustainable tourism development (STD) and SDG-5 attainment. The study research hypothesizes that women’s entrepreneurship positively influences empowerment, which in turn affects STD, tourism involvement, and support. Using an online survey, the study collected primary data from women in the Bačko Podunavlje region, part of the Transboundary Biosphere Reserve “Mura-Drava-Danube”. It employed structural equation modeling (SEM) to test its hypotheses. The results indicate that women’s entrepreneurship significantly contributes to empowerment, subsequently enhances sustainable tourism outcomes. The findings emphasize the need for community members and policymakers to design and implement strategies that reduce gender inequality and foster sustainable development. This study contributes to the literature on women’s empowerment and sustainable tourism in biosphere reserves and offers actionable insights for promoting gender equality through tourism.</p>