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<p>Browning of explants is a primary challenge on mango callus. However, some mango embryos remained un-browned upon inoculation. Bacterial plaques appeared around them after two days of culture. Isolation of endophytes from such embryos revealed that the dominant genera were <i>Pantoea</i> and <i>Azospirillum</i>. The bacterial plaques were cultured and centrifuged. The supernatant was filtered and added into the callus-induction medium. The browning area of mango embryos cultured on medium containing the filtered supernatant was about 5% of that cultured on control medium. Adding citric acid or GSH into the medium inhibited the browning of mango immature embryos, though less effectively than supplementing with the filtered supernatant of the endophyte culture. This study provides a method to inhibit the browning of mango immature embryos when callus was induced, laying a foundation for mango tissue culture and transgenic experiments.</p>

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The inhibition of browning of mango embryos by endophytes

  • 期刊论文

  • 发表日期：

    2026年4月14日

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  • Haiyan Shu,
  • Qing Wei,
  • Songbiao Wang,
  • Rulin Zhan,
  • Shenghe Chang

摘要

Browning of explants is a primary challenge on mango callus. However, some mango embryos remained un-browned upon inoculation. Bacterial plaques appeared around them after two days of culture. Isolation of endophytes from such embryos revealed that the dominant genera were Pantoea and Azospirillum. The bacterial plaques were cultured and centrifuged. The supernatant was filtered and added into the callus-induction medium. The browning area of mango embryos cultured on medium containing the filtered supernatant was about 5% of that cultured on control medium. Adding citric acid or GSH into the medium inhibited the browning of mango immature embryos, though less effectively than supplementing with the filtered supernatant of the endophyte culture. This study provides a method to inhibit the browning of mango immature embryos when callus was induced, laying a foundation for mango tissue culture and transgenic experiments.