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<p>This study aims to investigate the effectiveness of an encapsulated powder containing savory essential oil and calcium propionate salt on reactive oxygen species (ROS) and the expression of antioxidant genes (<i>SOD</i>, <i>APX</i>, <i>CAT</i>) in pear seedlings (cv. Spadona) inoculated with <i>E. amylovora</i> (ATCC 49946). To this end, the antioxidant activity of 7 types of herbal essential oils with 10 types of different organic acid salts were evaluated in the laboratory. The most effective candidates (calcium propionate salt + <i>S. hortensis</i> essential oil) were encapsulated in maltodextrin and modified starch by the spray drying technique. To evaluate the efficiency of the encapsulated powder, a greenhouse experiment was designed using a Randomized Complete Block Design (RCBD) with six treatments (T<sub>1</sub>: 1&#xa0;g/L encapsulated powder, T<sub>2</sub>: 2&#xa0;g/L encapsulated powder, T<sub>3</sub>: 3&#xa0;g/L encapsulated powder, streptomycin (positive control, con. 100&#xa0;ppm), control: no inoculation and no foliar spray treatments, and infected: seedlings inoculated with a concentration of 10^7&#xa0;cfu/mL of <i>E. amylovora</i>). The findings demonstrated that increasing the concentration of the encapsulated powder treatment led to a relative increase in the expression of the antioxidant genes (<i>SOD</i>, <i>APX</i>, <i>CAT</i>) compared to the reference gene (<i>GAPDH</i>). The highest effect on <i>SOD</i>, <i>APX</i>, and <i>CAT</i> gene expression was seen in the third treatment (T<sub>3</sub>). The T<sub>1</sub>, T<sub>2</sub>, and T<sub>3</sub> treatments decreased the amount of Malondialdehyde (MDA), and led to an increase in total phenolic content (TPC) in pear leaves compared to the control treatment.</p>

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Improving the expression of antioxidant genes in pear seedlings inoculated with Erwinia amylovora through encapsulated bioactive compounds

  • 期刊论文

  • 发表日期：

    2026年1月28日

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  • Rouhollah Faramarzi-Dozein,
  • Elaheh Motamedi,
  • Saeed Tarighi,
  • Ehsan Oskoueian,
  • Aram Bostan

摘要

This study aims to investigate the effectiveness of an encapsulated powder containing savory essential oil and calcium propionate salt on reactive oxygen species (ROS) and the expression of antioxidant genes (SOD, APX, CAT) in pear seedlings (cv. Spadona) inoculated with E. amylovora (ATCC 49946). To this end, the antioxidant activity of 7 types of herbal essential oils with 10 types of different organic acid salts were evaluated in the laboratory. The most effective candidates (calcium propionate salt + S. hortensis essential oil) were encapsulated in maltodextrin and modified starch by the spray drying technique. To evaluate the efficiency of the encapsulated powder, a greenhouse experiment was designed using a Randomized Complete Block Design (RCBD) with six treatments (T1: 1 g/L encapsulated powder, T2: 2 g/L encapsulated powder, T3: 3 g/L encapsulated powder, streptomycin (positive control, con. 100 ppm), control: no inoculation and no foliar spray treatments, and infected: seedlings inoculated with a concentration of 10^7 cfu/mL of E. amylovora). The findings demonstrated that increasing the concentration of the encapsulated powder treatment led to a relative increase in the expression of the antioxidant genes (SOD, APX, CAT) compared to the reference gene (GAPDH). The highest effect on SOD, APX, and CAT gene expression was seen in the third treatment (T3). The T1, T2, and T3 treatments decreased the amount of Malondialdehyde (MDA), and led to an increase in total phenolic content (TPC) in pear leaves compared to the control treatment.