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<p>The efficacy of biocontrol agents (BCA’s) <i>Trichoderma harzianum</i> and <i>Bacillus amyloliquefaciens</i> with a hormonal elicitor like salicylic acid were screened&#xa0;for systemic resistance inducuction in melon against <i>Monosporascus cannonballus</i> in pots experiments. Preventive treatments were applied by watering melon (cv. Badii) seven days prior to <i>M. cannonballus</i> with artificial inoculum. The two controls were (i) untreated and healthy plants as negative control and (ii) only inoculated as positive controls. Agronomic traits, biochemical activities (total phenol, flavonoids, catalase and peroxidase) from fresh roots and leaves, along with, pathogenesis-related (PR) protein gene expression from leaves was determined. All applied treatments significantly decreased the severity of the root disease index. This parameter was slightly lower in melon treated with <i>B. amyloliquefaciens</i> (0.33 at 45 DPI). These results were confirmed with an improvement for all agronomic parameters compared to the negative control. A stimulation of the phenolic content (polyphenols and flavonoids) and antioxidant enzymes (catalase and peroxidase) was monitored. Analysis of gene expression profiles (CmPR-1, CmPR-2, CmPR-3, CmPR-4 and CmPR-5) by real-time PCR has demonstrated the involvement of these genes in the development of plant defense against <i>M. cannonballus.</i> The variation of their expression depends on the treatment responsible for triggering the resistance reactions. This is the first association of total phenol, flavonoids, catalase and peroxidase and proteins PR gene expression with disease resistance to <i>M. cannonballus</i>.</p>

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Biochemical and genetic mechanisms of melon defense induction using Trichoderma harzianum, Bacillus amyloliquefaciens and salycilic acid against monosporascus root rot and vine decline

  • 期刊论文

  • 发表日期：

    2026年1月3日

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  • Ibtissem Ben Salem,
  • Sabrine Mannai,
  • Bouallegue Marwa,
  • Mahmoud M’Hamdi,
  • Naima Boughalleb-M.’Hamdi

摘要

The efficacy of biocontrol agents (BCA’s) Trichoderma harzianum and Bacillus amyloliquefaciens with a hormonal elicitor like salicylic acid were screened for systemic resistance inducuction in melon against Monosporascus cannonballus in pots experiments. Preventive treatments were applied by watering melon (cv. Badii) seven days prior to M. cannonballus with artificial inoculum. The two controls were (i) untreated and healthy plants as negative control and (ii) only inoculated as positive controls. Agronomic traits, biochemical activities (total phenol, flavonoids, catalase and peroxidase) from fresh roots and leaves, along with, pathogenesis-related (PR) protein gene expression from leaves was determined. All applied treatments significantly decreased the severity of the root disease index. This parameter was slightly lower in melon treated with B. amyloliquefaciens (0.33 at 45 DPI). These results were confirmed with an improvement for all agronomic parameters compared to the negative control. A stimulation of the phenolic content (polyphenols and flavonoids) and antioxidant enzymes (catalase and peroxidase) was monitored. Analysis of gene expression profiles (CmPR-1, CmPR-2, CmPR-3, CmPR-4 and CmPR-5) by real-time PCR has demonstrated the involvement of these genes in the development of plant defense against M. cannonballus. The variation of their expression depends on the treatment responsible for triggering the resistance reactions. This is the first association of total phenol, flavonoids, catalase and peroxidase and proteins PR gene expression with disease resistance to M. cannonballus.