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<p>Sheath blight disease caused by <i>Rhizoctonia solani</i> has become one of the major threats to rice cultivation all over the world including India. In this study, differential behaviour of <i>R. solani</i> among tolerant (Tetep, Wazuhophek and Ngonolosa) and susceptible rice cultivars (IR 50, and TN1) was analyzed at different stages of infection. Morphological and histopathological evaluations revealed that the growth and density of fungal hyphae, formation of infection cushions on the leaves, and formation of microsclerotia were significantly higher on susceptible cultivars, compared to tolerant cultivars. Biochemical assays showed that defence pathways were activated in tolerant and susceptible rice plants post-inoculation with <i>R. solani</i> at different levels and this was strongly correlated with the difference in disease severity. Induction of defence enzymes such as superoxide dismutase (SOD), peroxidase (POX) and polyphenol oxidase (PPO) were observed to be varying among the tolerant and susceptible cultivars, 120&#xa0;h post infection. Total protein content of tolerant cultivars was significantly higher compared to that of susceptible cultivars, post inoculation. Quantitative Real-Time PCR (qRT-PCR) analysis with resistant (Wazuhophek) and susceptible (IR 50) rice cultivars showed significant variation in expression levels of 12 defence related genes post inoculation. Wazuhophek had the highest level of defence gene expression post infection, possibly playing a key role in restricting pathogen growth and disease development.</p>

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Differential response of tolerant and susceptible cultivars of rice against sheath blight pathogen, Rhizoctonia solani Kühn

  • 期刊论文

  • 发表日期：

    2025年2月17日

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  • Puli Sasanka Roy,
  • Vellaichamy Prakasam,
  • Anil S. Kotasthane,
  • Prasanth Pranathi,
  • Chilumula Priyanka,
  • Vadakkenchery Mohanan Manoj,
  • Durbha Sanjeeva Rao,
  • Gouri Sankar Laha,
  • M Srinivas Prasad,
  • Jyothi Badri,
  • Satendra Kumar Mangrauthia,
  • Raman Meenakshi Sundaram

摘要

Sheath blight disease caused by Rhizoctonia solani has become one of the major threats to rice cultivation all over the world including India. In this study, differential behaviour of R. solani among tolerant (Tetep, Wazuhophek and Ngonolosa) and susceptible rice cultivars (IR 50, and TN1) was analyzed at different stages of infection. Morphological and histopathological evaluations revealed that the growth and density of fungal hyphae, formation of infection cushions on the leaves, and formation of microsclerotia were significantly higher on susceptible cultivars, compared to tolerant cultivars. Biochemical assays showed that defence pathways were activated in tolerant and susceptible rice plants post-inoculation with R. solani at different levels and this was strongly correlated with the difference in disease severity. Induction of defence enzymes such as superoxide dismutase (SOD), peroxidase (POX) and polyphenol oxidase (PPO) were observed to be varying among the tolerant and susceptible cultivars, 120 h post infection. Total protein content of tolerant cultivars was significantly higher compared to that of susceptible cultivars, post inoculation. Quantitative Real-Time PCR (qRT-PCR) analysis with resistant (Wazuhophek) and susceptible (IR 50) rice cultivars showed significant variation in expression levels of 12 defence related genes post inoculation. Wazuhophek had the highest level of defence gene expression post infection, possibly playing a key role in restricting pathogen growth and disease development.