Scholarlink

<p>Biochar is an effective adsorbent for antibiotics, but the impacts and mechanisms of biochar on oilseed rape (<i>Brassica napus</i> L.) under antibiotics stress are not yet clear. Therefore, we investigated root morphology, photosynthesis, root antioxidant systems, and root transcriptome treated by sulfamethazine (K) and sulfamethazine + <i>Platanus orientalis</i> L. leaf biochar (TK). The results indicated that the sulfamethazine stress decreased root length, surface area, volume, and diameter by 14.06%, 35.91%, 36.71%, and 19.47%, respectively, compared with CK because net photosynthetic rate was decreased by 35.54% and reactive oxygen species (H<sub>2</sub>O<sub>2</sub> and O<sub>2</sub><sup>−</sup>) balance was damaged. However application of the biochar increased net photosynthetic rate (38.84%), and further enhanced activity of catalase (27.48%) to decrease reactive oxygen species content, thereby promoting root growth, especially root length significantly increased by 29.55%. Transcriptomic analysis revealed that biochar induced root growth and reactive oxygen species balance of oilseed rape under sulfamethazine stress through enhancing the expression of genes related to the cell wall and auxin biosynthesis such as auxin response factor, auxin/indole-3-acetic acid, small auxin-up RNA, Gretchen-Hagen 3, and dormancy/auxin associated protein. Our study provides insights for the biochar improving oilseed rape growth under sulfamethazine stress, and can promote the remediation of antibiotic-contaminated farmland soil, agricultural production.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Physiological and transcriptome analysis reveal the important role of cell wall and auxin synthesis induced by biochar on oilseed rape root under sulfamethazine stress

  • 期刊论文

  • 发表日期：

    2026年1月20日

查看全文
  • Qilei Li,
  • Qiong Lu,
  • Lu Wang,
  • Enzhe Hou,
  • Juyuan Wang,
  • Sheng Zhai

摘要

Biochar is an effective adsorbent for antibiotics, but the impacts and mechanisms of biochar on oilseed rape (Brassica napus L.) under antibiotics stress are not yet clear. Therefore, we investigated root morphology, photosynthesis, root antioxidant systems, and root transcriptome treated by sulfamethazine (K) and sulfamethazine + Platanus orientalis L. leaf biochar (TK). The results indicated that the sulfamethazine stress decreased root length, surface area, volume, and diameter by 14.06%, 35.91%, 36.71%, and 19.47%, respectively, compared with CK because net photosynthetic rate was decreased by 35.54% and reactive oxygen species (H2O2 and O2−) balance was damaged. However application of the biochar increased net photosynthetic rate (38.84%), and further enhanced activity of catalase (27.48%) to decrease reactive oxygen species content, thereby promoting root growth, especially root length significantly increased by 29.55%. Transcriptomic analysis revealed that biochar induced root growth and reactive oxygen species balance of oilseed rape under sulfamethazine stress through enhancing the expression of genes related to the cell wall and auxin biosynthesis such as auxin response factor, auxin/indole-3-acetic acid, small auxin-up RNA, Gretchen-Hagen 3, and dormancy/auxin associated protein. Our study provides insights for the biochar improving oilseed rape growth under sulfamethazine stress, and can promote the remediation of antibiotic-contaminated farmland soil, agricultural production.