<p>The methods used in animal studies can influence organism responses in subtle ways. Toxicology studies conduct exposures on organisms individually or in groups, dependant on the goals of the study. It is important that the subtle influences of these choices in methodology are understood so that cross-study comparisons can be made within the context that the data was generated in. This study compared differences in toxicity and organism physiology in <i>Daphnia magna</i> exposed to ultraviolet filters across five generations between individual and group exposure scenarios. The baseline mortality of daphnids was doubled in group exposures, accompanied by an increasing number of males over subsequent generations to a maximum of 10% of the population, as well as delayed timing of maturation and brood frequency compared to individual organisms exposed with the same ratio of food and water. These differences were largely independent of chemical treatment, indicating that the presence of conspecifics influences the responses of <i>D. magna</i> across a variety of physiological endpoints. This study highlights the importance of experimental design when comparing toxicity data across studies so that confounding factors such as conspecific presence do not lead to inaccurate comparisons of data obtained across studies using differing methodologies.</p>