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<p>Enchytraeids (potworms) are widely-distributed oligochaetes highly useful as bioindicators of soil quality and for ecotoxicological tests to assess non-target effects of contaminants. However, the standard and most widely-used species (<i>Enchytraeus albidus</i> and <i>Enchytraeus crypticus</i>) are not well representative of potworms living in agricultural soils subjected to pesticide use in temperate and tropical regions. Fragmenting species like <i>Enchytraeus bigeminus</i> may be more ecologically relevant, and their rapid reproduction mode (fragmentation) may reduce assay time, offering faster results and thus more economical testing. In the present study we provide first results of ecotoxicological tests using the reference substance boric acid and the regeneration of <i>E. begeminus</i> fragments as a new end-point. Seven concentrations of boric acid were tested with five replicates per treatment. Decreases in regeneration after 7 days exposure were observed with higher concentrations of boric acid and the EC<sub>50</sub> value was 9.12&#xa0;mg kg<sup>-1</sup>. Regeneration was easily visible and rapidly assessed using a Ludox<sup>®</sup> and white paint solution, making this fragmenting species and endpoint particularly interesting for further standardization efforts.</p>

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Using fragmenting enchytraeid species in ecotoxicological tests: regeneration as a new endpoint

  • 期刊论文

  • 发表日期：

    2025年9月11日

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  • Orlando Assis,
  • Cintia C. Niva,
  • George G. Brown

摘要

Enchytraeids (potworms) are widely-distributed oligochaetes highly useful as bioindicators of soil quality and for ecotoxicological tests to assess non-target effects of contaminants. However, the standard and most widely-used species (Enchytraeus albidus and Enchytraeus crypticus) are not well representative of potworms living in agricultural soils subjected to pesticide use in temperate and tropical regions. Fragmenting species like Enchytraeus bigeminus may be more ecologically relevant, and their rapid reproduction mode (fragmentation) may reduce assay time, offering faster results and thus more economical testing. In the present study we provide first results of ecotoxicological tests using the reference substance boric acid and the regeneration of E. begeminus fragments as a new end-point. Seven concentrations of boric acid were tested with five replicates per treatment. Decreases in regeneration after 7 days exposure were observed with higher concentrations of boric acid and the EC50 value was 9.12 mg kg-1. Regeneration was easily visible and rapidly assessed using a Ludox® and white paint solution, making this fragmenting species and endpoint particularly interesting for further standardization efforts.