<p>In eastern Canada, brook charr (<i>Salvelinus fontinalis</i>) support a strong sport fishing industry that depends on captive breeding programs to maintain its sustainability. Many local communities depend on this industry, and maintaining the welfare and success of stocked fish is a priority. Identifying the behavioral profiles of fish before release has been used to predict post-stocking survival. In this study, we compared behavioral traits between fry (6-months post-fertilization) issued from two groups of brook charr breeders sourced from the wild: (1) breeders captured in a lake where there has been no previous stocking activity—“wild”; (2) breeders originating from stocked fry in lakes from the same area, but in which sport fishing is supported by stocking activities—“stocked”. Eggs and larvae were incubated and reared under the same environmental conditions in the hatchery. One hundred and sixty-eight fry from each group underwent a 10-min behavioral assay during which they were subjected to open-field, mirror- and anti-predator tests. Fry issued from stocked breeders were bolder and more aggressive (<i>p</i> &lt; 0.001) than those issued from wild breeders, while wild-sourced offspring exhibited greater avoidance behaviors (<i>p</i> &lt; 0.001). The antipredator response was similar between the two groups. The persistence of these behavioral differences across lakes may have significant ecological consequences for survival and resilience in natural habitats after stocking. Understanding these profiles is an important step to enhancing the effectiveness of stocking programs for recreational fishing.</p>