Scholarlink

<p>Temperature is a critical environmental factor that affects animal physiological activities and behaviors. Both acute and chronic temperature changes can affect normal activities in animals. However, the extent to which different temperatures influence animal fear responses, and the underlying regulatory mechanisms, remains largely unknown. In this study, male zebrafish, <i>Danio rerio</i> (Hamilton, 1822), were exposed to different temperatures (Blank and Control, 27&#xa0;°C; low temperature, L–T, 20&#xa0;°C; high temperature, H-T, 34&#xa0;°C) for 7&#xa0;days. After treatment, the fish were subjected to novel tank diving tests using distilled water (DW, Blank) or alarm substance (AS, Control, L–T, H-T) stimuli, after which we recorded the following fear parameters: the onset time to higher half, duration in lower half, and duration of freezing. Our data indicated that L–T increased fear responses in male zebrafish, whereas H-T decreased fear responses in male zebrafish. Measurements of relevant hormones and neurotransmitters revealed that L–T elevated plasma cortisol levels as well as norepinephrine (NE), serotonin (5-HT), and dopamine (DA) levels in the brain, whereas H-T reduced plasma cortisol and brain NE levels, while increasing brain 5-HT and DA levels. Additionally, the expression of genes related to the hypothalamus-pituitary-interrenal (HPI) axis and the NE/5-HT/DAergic systems in fish changed accordingly. Our data suggest that subacute exposure to L–T may enhance the AS-induced fear response by stimulating the HPI/cortisol axis and the NEergic system, while H-T may alleviate the AS-induced fear response by inhibiting the HPI/cortisol axis and the NEergic system, while enhancing the 5-HT/DAergic systems.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Subacute temperature stress altered the fear response of male zebrafish by disrupting HPI/cortisol axis and NE/5-HT/DA neurotransmitter systems

  • 期刊论文

  • 发表日期：

    2025年6月7日

查看全文
  • Hong Tao,
  • Ying–Ying Zhang,
  • Yan–Jun Shen,
  • Qi–Liang Chen,
  • Zhi–Hao Liu

摘要

Temperature is a critical environmental factor that affects animal physiological activities and behaviors. Both acute and chronic temperature changes can affect normal activities in animals. However, the extent to which different temperatures influence animal fear responses, and the underlying regulatory mechanisms, remains largely unknown. In this study, male zebrafish, Danio rerio (Hamilton, 1822), were exposed to different temperatures (Blank and Control, 27 °C; low temperature, L–T, 20 °C; high temperature, H-T, 34 °C) for 7 days. After treatment, the fish were subjected to novel tank diving tests using distilled water (DW, Blank) or alarm substance (AS, Control, L–T, H-T) stimuli, after which we recorded the following fear parameters: the onset time to higher half, duration in lower half, and duration of freezing. Our data indicated that L–T increased fear responses in male zebrafish, whereas H-T decreased fear responses in male zebrafish. Measurements of relevant hormones and neurotransmitters revealed that L–T elevated plasma cortisol levels as well as norepinephrine (NE), serotonin (5-HT), and dopamine (DA) levels in the brain, whereas H-T reduced plasma cortisol and brain NE levels, while increasing brain 5-HT and DA levels. Additionally, the expression of genes related to the hypothalamus-pituitary-interrenal (HPI) axis and the NE/5-HT/DAergic systems in fish changed accordingly. Our data suggest that subacute exposure to L–T may enhance the AS-induced fear response by stimulating the HPI/cortisol axis and the NEergic system, while H-T may alleviate the AS-induced fear response by inhibiting the HPI/cortisol axis and the NEergic system, while enhancing the 5-HT/DAergic systems.