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<p>In this study, an innovative rotating-drum bioreactor was evaluated and compared with a conventional stirred-tank system for CHO cell fed-batch culture, with particular focus on oxygen transfer, hydrodynamic conditions, and overall bioprocess performance. The novel configuration, based on a slowly rotating perforated drum, enabled efficient oxygenation at low rotational speeds, achieving a higher volumetric mass transfer coefficient (k<sub>L</sub>a = 0.1087&#xa0;min⁻¹) despite significantly lower tip speeds. This resulted in stable dissolved oxygen (DO) levels (approximately 40%) throughout a 10-day cultivation. Under these operating conditions, the innovative bioreactor promoted enhanced cell growth, achieving a peak viable cell density (VCD) of 2.55 × 10⁷ cells mL⁻¹ and maintaining high viability (around 91.7% on day 10). Monoclonal antibody production reached 1.3 ± 0.09&#xa0;g L⁻¹, almost doubling the yield obtained in the stirred-tank reactor. The improved outcomes observed in the innovative bioreactor could be associated with the distinct operating and hydrodynamic conditions established by the system configuration. Nevertheless, further studies are needed to better define the operational limits of the system and optimize process conditions. Future investigations should also assess critical quality attributes, including glycosylation patterns, aggregation, fragmentation, and host cell protein contamination, as well as evaluate the performance of the bioreactor at larger scales.</p> Graphical abstract <p></p>

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Evaluation of a prototype bioreactor based on slowly rotating drum

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月17日

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  • Alfredo Ambrico,
  • Mario Trupo,
  • Rosaria Alessandra Magarelli,
  • Vincenzo Larocca,
  • Maria Martino,
  • Sergio Modenese,
  • Sirio Vurro

摘要

In this study, an innovative rotating-drum bioreactor was evaluated and compared with a conventional stirred-tank system for CHO cell fed-batch culture, with particular focus on oxygen transfer, hydrodynamic conditions, and overall bioprocess performance. The novel configuration, based on a slowly rotating perforated drum, enabled efficient oxygenation at low rotational speeds, achieving a higher volumetric mass transfer coefficient (kLa = 0.1087 min⁻¹) despite significantly lower tip speeds. This resulted in stable dissolved oxygen (DO) levels (approximately 40%) throughout a 10-day cultivation. Under these operating conditions, the innovative bioreactor promoted enhanced cell growth, achieving a peak viable cell density (VCD) of 2.55 × 10⁷ cells mL⁻¹ and maintaining high viability (around 91.7% on day 10). Monoclonal antibody production reached 1.3 ± 0.09 g L⁻¹, almost doubling the yield obtained in the stirred-tank reactor. The improved outcomes observed in the innovative bioreactor could be associated with the distinct operating and hydrodynamic conditions established by the system configuration. Nevertheless, further studies are needed to better define the operational limits of the system and optimize process conditions. Future investigations should also assess critical quality attributes, including glycosylation patterns, aggregation, fragmentation, and host cell protein contamination, as well as evaluate the performance of the bioreactor at larger scales.

Graphical abstract