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<p>Dendrobine exhibits notable anti-tumor activity against breast cancer (BRCA). In this study, we integrated network pharmacology, bioinformatics, and experimental validation to elucidate its mechanisms. SwissTargetPrediction and differential gene analyses identified potential dendrobine targets, highlighting apoptosis-related pathways and implicating SLC6A9 as a key prognostic gene associated with cell cycle regulation and NF-κB signaling. Molecular docking further demonstrated strong binding affinities between dendrobine and SLC6A9/NF-κB. In vitro experiments using MDA-MB-231 cells confirmed that dendrobine suppresses proliferation and migration in a concentration-dependent manner, as shown by CCK-8 and wound-healing assays, while significantly inducing apoptosis, verified through flow cytometry. Western blot analysis revealed downregulation of proliferation- and invasion-related proteins (PCNA, Cyclin D1) and activation of pro-apoptotic markers (BAX, cleaved-caspase3), accompanied by inhibition of NF-κB pathway activation. Collectively, these findings demonstrate that dendrobine exerts anti-BRCA effects primarily by downregulating SLC6A9 and suppressing NF-κB signaling, supporting its potential as a natural therapeutic candidate for breast cancer.</p>

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Dendrobine inhibits the growth and invasion of human breast cancer cells by regulating the NF-κB signaling pathway via the SLC6A9 target

  • 期刊论文

  • 发表日期：

    2026年5月16日

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  • Shaoli Xie,
  • Bijuan Wang,
  • Hongying Zhang,
  • Jianping Yang,
  • Lulan Pu,
  • Jiayou Liu

摘要

Dendrobine exhibits notable anti-tumor activity against breast cancer (BRCA). In this study, we integrated network pharmacology, bioinformatics, and experimental validation to elucidate its mechanisms. SwissTargetPrediction and differential gene analyses identified potential dendrobine targets, highlighting apoptosis-related pathways and implicating SLC6A9 as a key prognostic gene associated with cell cycle regulation and NF-κB signaling. Molecular docking further demonstrated strong binding affinities between dendrobine and SLC6A9/NF-κB. In vitro experiments using MDA-MB-231 cells confirmed that dendrobine suppresses proliferation and migration in a concentration-dependent manner, as shown by CCK-8 and wound-healing assays, while significantly inducing apoptosis, verified through flow cytometry. Western blot analysis revealed downregulation of proliferation- and invasion-related proteins (PCNA, Cyclin D1) and activation of pro-apoptotic markers (BAX, cleaved-caspase3), accompanied by inhibition of NF-κB pathway activation. Collectively, these findings demonstrate that dendrobine exerts anti-BRCA effects primarily by downregulating SLC6A9 and suppressing NF-κB signaling, supporting its potential as a natural therapeutic candidate for breast cancer.