Scholarlink

<p>Marine-derived functional ingredients are gaining attention for their therapeutic potential in skin health and inflammation. The wound healing and anti-inflammatory properties of <i>Holothuria atra</i>, an edible sea cucumber, remain underexplored. This study aimed to evaluate the in vitro wound healing activity of <i>H. atra</i> methanol extract and the in silico anti–tumor necrosis factor-<i>α</i> (TNF-<i>α</i>) of its metabolites. Cytotoxicity on human keratinocyte (HaCaT) cells was assessed, followed by scratch assays under normal and TNF-<i>α</i>-induced conditions. The components of the extract were identified based on their molecular masses, and key metabolites were docked against TNF-<i>α</i> binding sites. The extract showed 83.8 ± 2.5% cell viability at 100 ng/mL, which was used as the maximum concentration. In the scratch assay, wound closure increased with dose, reaching 53.2 ± 8.8% after 48&#xa0;h at 100 ng/mL, compared to 18.4 ± 6.9% in the control (<i>p</i> &lt; 0.0001). With TNF-<i>α</i> (10 ng/mL), cell migration dropped (1.0 ± 0.2% after 48&#xa0;h); however, 100 ng/mL extract restored closure to 53.2 ± 15.1% (<i>p</i> &lt; 0.001) after 48&#xa0;h. GC-MS revealed major fatty acids, including arachidonic (17.5%) and palmitic acid (15.2%), while LC-MS identified ten saponins, six verified by standards. Docking showed metabolite binding to TNF-<i>α</i> (–9.29 to − 7.47&#xa0;kcal/mol), particularly at Tyr119. In conclusion, <i>H. atra</i> methanol extract enhanced keratinocyte wound healing in vitro, and its metabolites showed TNF-<i>α</i> binding ability based on the molecular docking study. However, further in vivo validation is warranted.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Anti-inflammatory and skin-regenerative properties of Red Sea Holothuria atra: in vitro wound healing activity of the methanolic extract and in silico anti–Tumor Necrosis Factor-α evaluation of its bioactive metabolites

  • 期刊论文

  • 发表日期：

    2025年12月12日

查看全文
  • Amira Elkattan,
  • Akiko Isa,
  • Dongmei Wang,
  • Aya M. Almatary,
  • Ahmed Othman,
  • Kuniyoshi Shimizu

摘要

Marine-derived functional ingredients are gaining attention for their therapeutic potential in skin health and inflammation. The wound healing and anti-inflammatory properties of Holothuria atra, an edible sea cucumber, remain underexplored. This study aimed to evaluate the in vitro wound healing activity of H. atra methanol extract and the in silico anti–tumor necrosis factor-α (TNF-α) of its metabolites. Cytotoxicity on human keratinocyte (HaCaT) cells was assessed, followed by scratch assays under normal and TNF-α-induced conditions. The components of the extract were identified based on their molecular masses, and key metabolites were docked against TNF-α binding sites. The extract showed 83.8 ± 2.5% cell viability at 100 ng/mL, which was used as the maximum concentration. In the scratch assay, wound closure increased with dose, reaching 53.2 ± 8.8% after 48 h at 100 ng/mL, compared to 18.4 ± 6.9% in the control (p < 0.0001). With TNF-α (10 ng/mL), cell migration dropped (1.0 ± 0.2% after 48 h); however, 100 ng/mL extract restored closure to 53.2 ± 15.1% (p < 0.001) after 48 h. GC-MS revealed major fatty acids, including arachidonic (17.5%) and palmitic acid (15.2%), while LC-MS identified ten saponins, six verified by standards. Docking showed metabolite binding to TNF-α (–9.29 to − 7.47 kcal/mol), particularly at Tyr119. In conclusion, H. atra methanol extract enhanced keratinocyte wound healing in vitro, and its metabolites showed TNF-α binding ability based on the molecular docking study. However, further in vivo validation is warranted.