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<p>Different extract products of <i>Capparis ovata</i> species have antimicrobial, antidiabetic, antineuroinflammatory, and also hepatotoxicity and oxidative stress inhibitory effects. In our study, we aimed to compare the cytotoxic effects of polysaccharides isolated from <i>Capparis ovata</i>, on two different colon cancer cell lines by VEGF and GSK-3β signaling. Caco-2 and HT-29 colon cancer cells were treated with <i>Capparis ovata</i> polysaccharides and doxorubicin at varied concentrations for 24 and 48&#xa0;h. The proliferation levels were measured by MTT assay, expression of VEGF and GSK-3β molecules determined using qRT-PCR assay and immunocytochemical staining. And the scratch wound model was used the determined the effect of polysaccharides on cell migration. The data was analyzed statistically. It was found that cell proliferation and migration were suppressed by <i>Capparis ovata</i> polysaccharides concentration dependent manner. Also <i>Capparis ovata</i> polysaccharides significantly decreased the expression of VEGF and GSK-3β molecules in both cells compared with the untreated cells. According to our findings, <i>Capparis ovata</i> polysaccharides showed cytotoxic effect on Caco-2 and HT-29 cells concentration dependent manner, by inhibiting the expression of VEGF and GSK-3β which play a role in tumor formation and metastasis. So, cell migration was repressed.</p>

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Assessment for cytotoxic properties of Capparis ovata polysaccharides in Caco-2 and HT29 colon cancer cells via VEGF and GSK-3β signaling

  • 期刊论文

  • 发表日期：

    2025年9月8日

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  • Işıl Aydemir,
  • Emre Çetindağ,
  • Esma Özmen,
  • Serpil Demirci Kayıran,
  • Derya Deniz Kanan,
  • Esra Sakallı,
  • Selim Çınaroğlu,
  • Gonca Dönmez Arat,
  • Oktay Özkan

摘要

Different extract products of Capparis ovata species have antimicrobial, antidiabetic, antineuroinflammatory, and also hepatotoxicity and oxidative stress inhibitory effects. In our study, we aimed to compare the cytotoxic effects of polysaccharides isolated from Capparis ovata, on two different colon cancer cell lines by VEGF and GSK-3β signaling. Caco-2 and HT-29 colon cancer cells were treated with Capparis ovata polysaccharides and doxorubicin at varied concentrations for 24 and 48 h. The proliferation levels were measured by MTT assay, expression of VEGF and GSK-3β molecules determined using qRT-PCR assay and immunocytochemical staining. And the scratch wound model was used the determined the effect of polysaccharides on cell migration. The data was analyzed statistically. It was found that cell proliferation and migration were suppressed by Capparis ovata polysaccharides concentration dependent manner. Also Capparis ovata polysaccharides significantly decreased the expression of VEGF and GSK-3β molecules in both cells compared with the untreated cells. According to our findings, Capparis ovata polysaccharides showed cytotoxic effect on Caco-2 and HT-29 cells concentration dependent manner, by inhibiting the expression of VEGF and GSK-3β which play a role in tumor formation and metastasis. So, cell migration was repressed.