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<p>This study reports the first systematic isolation and characterization of bioactive peptides from <i>Cuminum cyminum</i> L. seeds using 50% ethanol extraction coupled with C18 reverse-phase chromatography. Three peptide fractions were evaluated for antimicrobial, antioxidant, and hypoglycemic activities. ZD-30 and ZD-50 exhibited potent antibacterial effects against <i>Escherichia coli</i> and significant antioxidant capacity, while ZD-70 demonstrated hypoglycemic potential via PTP1B inhibition. MALDI-TOF/TOF MS/MS analysis identified 479 unique peptide sequences, with 15 multifunctional candidates prioritized through computational tools (PeptideRanker, ToxinPred, PEP-FOLD3). Molecular dynamics simulations revealed α-helix/β-sheet conformations critical for membrane interactions and bioactivity. These findings validate <i>Cumin’s</i> traditional medicinal applications and provide a molecular foundation for developing plant-derived therapeutics targeting antimicrobial resistance and metabolic disorders.</p>

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Comprehensive Analysis of Bioactive Peptides from Cuminum cyminum Seeds: Sequence Identification and Pharmacological Evaluation

  • 期刊论文

  • 发表日期：

    2026年1月1日

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  • I. Ermatov,
  • S. Subuer,
  • N. Ullah,
  • H. Taurtaihon,
  • A. Wali,
  • Y. Mijiti

摘要

This study reports the first systematic isolation and characterization of bioactive peptides from Cuminum cyminum L. seeds using 50% ethanol extraction coupled with C18 reverse-phase chromatography. Three peptide fractions were evaluated for antimicrobial, antioxidant, and hypoglycemic activities. ZD-30 and ZD-50 exhibited potent antibacterial effects against Escherichia coli and significant antioxidant capacity, while ZD-70 demonstrated hypoglycemic potential via PTP1B inhibition. MALDI-TOF/TOF MS/MS analysis identified 479 unique peptide sequences, with 15 multifunctional candidates prioritized through computational tools (PeptideRanker, ToxinPred, PEP-FOLD3). Molecular dynamics simulations revealed α-helix/β-sheet conformations critical for membrane interactions and bioactivity. These findings validate Cumin’s traditional medicinal applications and provide a molecular foundation for developing plant-derived therapeutics targeting antimicrobial resistance and metabolic disorders.