Isolation, Purification, and Properties of Glycoprotein from Sheep Intestinal Mucous Membrane

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发表日期：

2024年1月30日

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Y. H. Wang,
G. Turdu,
X. M. Zhao,
A. L. Hu,
Z. Yang,
N. Dongmulati,
A. Arken,
R. Kelaimu,
A. Wali,
Y. Ali,
Y. H. Gao,
G. T. Mavlonov,
A. Yili,
H. A. Aisa

摘要

A glycoprotein inhibiting the growth of colon cancer cells was isolated from sheep intestinal mucous membrane. The glycoprotein, designated IGP, was purified by a combination of ion-exchange chromatography over DEAE-650M and gel-filtration over HW-55F. Purified IGP had a molecular mass of ~60 kDa and consisted of 68.72 ± 2.10% protein and 32.09 ± 1.52% carbohydrates. The monosaccharide composition of the carbohydrate part consisted of glucose (31.28% of the total carbohydrate content), glucosamine (22.28%) and N-acetylgalactosamine (19.38%), mannose (11.14%), fucose (11.28%), and rhamnose (4.64%). The amino-acid composition of IGP was also determined.