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<p>The present study examines an alternative approach for the production of Bacterial cellulose (BC) by <i>Enterobacter roggenkampii</i> IITISM CP-1 on modified sweet lime extract (SL) media. The research focuses on systematically optimizing abiotic factors in the natural media. The response surface methodology using a central composite design model was utilized to mathematically predict the influence of variation of pH, temperature, carbon, and nitrogen sources on BC production. The study suggests that the addition of external carbon sources, glucose (2.3%), nitrogen, yeast, and peptone in a ratio of 1:1 (4.25%) enhanced BC production. The optimum pH and temperature for the strain were predicted to be 6.1 and 30.3&#xa0;°C. The maximum BC yield was found to be 933.7&#xa0;mg/100&#xa0;mL in SL media in batch experiments under optimized parameters predicted by the model, which was found to be a more than three-fold increase compared to Hestrin and Schramm (HS) media under the same physical conditions. The produced cellulose showed a water holding capacity of 104% with crystallinity degree of 59.4% in X-ray diffraction (XRD) analysis and had similar properties to cellulose produced on HS media. The findings suggest that <i>Enterobacter roggenkampii</i> can serve as a potent strain for production of BC using semi-synthetic SL media.</p>

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Statistical modelling and optimization of bacterial cellulose formation by Enterobacter roggenkampii IITISM CP-1 in sweet lime extract media through response surface methodology

  • 期刊论文

  • 发表日期：

    2026年3月19日

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  • Nishant Pandey,
  • Vipin Kumar,
  • Ankur Singh,
  • Saumya Anand,
  • Dixita Phukan

摘要

The present study examines an alternative approach for the production of Bacterial cellulose (BC) by Enterobacter roggenkampii IITISM CP-1 on modified sweet lime extract (SL) media. The research focuses on systematically optimizing abiotic factors in the natural media. The response surface methodology using a central composite design model was utilized to mathematically predict the influence of variation of pH, temperature, carbon, and nitrogen sources on BC production. The study suggests that the addition of external carbon sources, glucose (2.3%), nitrogen, yeast, and peptone in a ratio of 1:1 (4.25%) enhanced BC production. The optimum pH and temperature for the strain were predicted to be 6.1 and 30.3 °C. The maximum BC yield was found to be 933.7 mg/100 mL in SL media in batch experiments under optimized parameters predicted by the model, which was found to be a more than three-fold increase compared to Hestrin and Schramm (HS) media under the same physical conditions. The produced cellulose showed a water holding capacity of 104% with crystallinity degree of 59.4% in X-ray diffraction (XRD) analysis and had similar properties to cellulose produced on HS media. The findings suggest that Enterobacter roggenkampii can serve as a potent strain for production of BC using semi-synthetic SL media.