<p>Nanotechnology is used on a large scale in the textile sector to improve fabrics or give them unique functions. Medical textiles used in healthcare settings require enhanced functional properties to protect healthcare workers from pathogenic bacteria and fluid contamination while maintaining comfort and durability. Medical textile products must meet stringent quality standards to ensure safety for both patients and healthcare workers in clinical environments worldwide. Therefore, to achieve certain functional properties like antibacterial and liquid-repellent qualities, silicon dioxide nanoparticles and chitosan nanoparticles were used to treat some knitted fabrics (viscose, lyocell, and viscose/lyocell blends) for use in creating medical gowns for doctors to protect them from the risks posed by bacteria and fluid. The results indicated that the best concentration of silicon dioxide nanoparticles was 3%, which recorded water contact angles of 154.6 ± 1.8 degrees, 155.3 ± 1.5 degrees, and 163.4 ± 1.3 degrees for viscose, viscose/lyocell blends, and lyocell fabrics, respectively. Fabrics treated with a weight concentration of 3% chitosan nanoparticles showed ideal bacterial resistance reaching 98.4 ± 0.5%, 98.7 ± 0.3%, and 99.1 ± 0.4% for gram-positive bacteria and 95.6 ± 0.6%, 96.1 ± 0.4%, and 97.2 ± 0.5% for gram-negative bacteria for viscose, lyocell, viscose/lyocell blends, respectively. This study demonstrates the successful development of multifunctional cellulosic textiles with combined antimicrobial and water-repellent properties for medical applications.</p>