Scholarlink

<p>The rarely reported nanocellulose micropheres (NCMs) are promising carriers for enzyme immobilization. Herein, the present study successfully prepared an enzyme immobilization system based on modified nanocellulose microspheres with porous structure. The electrostatic attraction and covalent bonding between polydopamine/polyethyleneimine (PDA/PEI) co-modified nanocellulose microspheres (NCMs) and -COOH/-NH<sub>2</sub> residues in the enzyme achieved the immobilization of β-glucosidase (PDA/PEI@NCMs@BGL). PDA/PEI@NCMs@BGL exhibited excellent stability and reusability with 91.41% residual enzyme activity after 5 cycles and converted at least 90.47% of geniposide, polydatin, and genistin at 4&#xa0;h. This study may lay a foundation for a cost-effective and environmentally friendly immobilized enzyme system based on modified nanocellulose for the bioconversion of phytochemicals.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A novel immobilization enzymatic system of nanocellulose-based β-glucosidase in O-glycoside bioconversion of phytochemicals

  • 期刊论文

  • 发表日期：

    2025年3月10日

查看全文
  • Jihong Cui,
  • Lina Fu,
  • Quan Zhou,
  • Jiaqi Zhang,
  • Xinlin Zhang,
  • Jie Yang,
  • Yujie Fu

摘要

The rarely reported nanocellulose micropheres (NCMs) are promising carriers for enzyme immobilization. Herein, the present study successfully prepared an enzyme immobilization system based on modified nanocellulose microspheres with porous structure. The electrostatic attraction and covalent bonding between polydopamine/polyethyleneimine (PDA/PEI) co-modified nanocellulose microspheres (NCMs) and -COOH/-NH2 residues in the enzyme achieved the immobilization of β-glucosidase (PDA/PEI@NCMs@BGL). PDA/PEI@NCMs@BGL exhibited excellent stability and reusability with 91.41% residual enzyme activity after 5 cycles and converted at least 90.47% of geniposide, polydatin, and genistin at 4 h. This study may lay a foundation for a cost-effective and environmentally friendly immobilized enzyme system based on modified nanocellulose for the bioconversion of phytochemicals.