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<p>Despite zygotic genome activation (ZGA) is crucial for early embryonic development, its regulatory mechanism is still unclear in mammals. In the present study, we demonstrate that TRPS1, a maternal factor, plays an essential role in mouse early embryogenesis by regulating the transition from 2-cell to 4-cell embryos during preimplantation development. The absence of <i>Trps1</i> could leads to impaired ZGA through AKT/CREB signaling pathway. Furthermore, our findings suggest that TRPS1 may modulate the transcription of <i>Pde4d</i> to influence AKT and CREB phosphorylation. Interestingly, compared to <i>Trps1</i> knockdown alone, co-injection of <i>Trps1</i> siRNA and <i>Pde4d</i> mRNA significantly enhances the development rate of 4-cell embryos. Collectively, these results indicate a negative involvement of <i>Trps1</i> in mouse preimplantation embryo development by targeting the PDE4D/AKT/CREB pathway to regulate ZGA.</p> Graphical Abstract <p></p> <p><UnorderedList Mark="None"> <ItemContent> <p><i>Trps1</i> regulate the transition from 2-cell to 4-cell embryos through AKT/CREB pathway.</p> </ItemContent> <ItemContent> <p>TRPS1 targets <i>Pde4d</i> to activation of AKT and CREB.</p> </ItemContent> <ItemContent> <p>TRPS1 is capable of combination with the promoter of the<i> Pde4d</i></p> </ItemContent> </UnorderedList></p>

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Trps1 regulates mouse zygotic genome activation and preimplantation embryo development via the PDE4D/AKT/CREB signaling pathway

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月20日

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  • Xia Jiang,
  • Weiwei Xu,
  • Jiandong Sun,
  • Jianmin Lin,
  • Zihang Lin,
  • Xiuli Lian,
  • Shumin Liao,
  • Shanshan Luo,
  • Yue Liu,
  • Shie Wang

摘要

Despite zygotic genome activation (ZGA) is crucial for early embryonic development, its regulatory mechanism is still unclear in mammals. In the present study, we demonstrate that TRPS1, a maternal factor, plays an essential role in mouse early embryogenesis by regulating the transition from 2-cell to 4-cell embryos during preimplantation development. The absence of Trps1 could leads to impaired ZGA through AKT/CREB signaling pathway. Furthermore, our findings suggest that TRPS1 may modulate the transcription of Pde4d to influence AKT and CREB phosphorylation. Interestingly, compared to Trps1 knockdown alone, co-injection of Trps1 siRNA and Pde4d mRNA significantly enhances the development rate of 4-cell embryos. Collectively, these results indicate a negative involvement of Trps1 in mouse preimplantation embryo development by targeting the PDE4D/AKT/CREB pathway to regulate ZGA.

Graphical Abstract

Trps1 regulate the transition from 2-cell to 4-cell embryos through AKT/CREB pathway.

TRPS1 targets Pde4d to activation of AKT and CREB.

TRPS1 is capable of combination with the promoter of the Pde4d