<p>In the present study, zeolite H-BEA catalyst has been modified by controlled desilication post modification route using first with cationic surfactant, (6-Bromohexyl)trimethylammonium Bromide (6-Br-TMA) designated as MBB catalyst and then the other with 6-Br-TMA and yeast, designated as MBBY catalyst. These newly synthesized catalytic materials have been characterized by several techniques such as, Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction, scanning electron microscopy, solid state NMR (²⁹Si, ²⁷Al, ¹H) N₂ sorption isotherm analysis, thermogravimetric analysis, ammonia temperature program desorption (NH₃-TPD) analysis, etc. These mesozeolites were found to exhibit enhanced physicochemical (BET surface area, surface acidity, pore volume, mesoporosity, etc.) characteristics as compared to their parent zeolite H-BEA. Catalytic assessement studies of the synthesized zeolites has been demonstrated via conducting experiments involving esterification of levulinic acid (LA) and <i>n</i>-hexanol. Parametric studies has been undertaken in order to get maximum LA conversion by varying several reaction parameters such as molar ratio (acid to <i>n</i>-hexanol), catalyst concentration, reaction time, etc. Further catalysts’ reusability studies has been carried out to assess their potential for reusability. The results indicated better catalytic performance of the zeolite H-BEA which is modified with the use of yeast (MBBY) as compared to&#xa0;microporous zeolite H-BEA and MBB catalyst which may be due to its enhanced surface area and surface acidity.</p> Graphical Abstract <p></p>