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<p>The present study aimed to evaluate the effectiveness of super-activated platelet lysate (sPL), as well as sPL in combination with bone marrow mesenchymal stem cells (BMSCs) in treating bone defects in rats. Ninety rats with induced radial defects were divided into three groups: sPL/BMSCs, sPL, and control. The healing process was evaluated using X-rays, Lane Sandhu scores, HE staining, RT-qPCR, immunohistochemistry, and ELISA. Both sPL and sPL/BMSCs facilitate bone repair. Compared to the other groups, the sPL/BMSCs group showed higher OCN, OSX, and RUNX2 gene expression until 10&#xa0;weeks after treatment ALP levels in the sPL group increased until week 6 and remained elevated until week 10, with the sPL/BMSCs group showing significantly higher peak ALP levels. IGF-1, TGF-β, and VEGF levels in both treatment groups were higher than in the control group. Our study demonstrated that both sPL and sPL/BMSC transplants at the bone defect site can promote bone healing and modulate bone metabolism-related factors. However, the effect of sPL/BMSCs was superior to sPL alone.</p>

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Effects of super-activated platelet lysate and bone mesenchymal stem cells on bone regeneration, and expression of bone metabolism-related factors in rats

  • 期刊论文

  • 发表日期：

    2025年10月4日

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  • Huiying Lu,
  • Tingting Li,
  • Junqi Liu,
  • Zitao Li,
  • Yi Zhang

摘要

The present study aimed to evaluate the effectiveness of super-activated platelet lysate (sPL), as well as sPL in combination with bone marrow mesenchymal stem cells (BMSCs) in treating bone defects in rats. Ninety rats with induced radial defects were divided into three groups: sPL/BMSCs, sPL, and control. The healing process was evaluated using X-rays, Lane Sandhu scores, HE staining, RT-qPCR, immunohistochemistry, and ELISA. Both sPL and sPL/BMSCs facilitate bone repair. Compared to the other groups, the sPL/BMSCs group showed higher OCN, OSX, and RUNX2 gene expression until 10 weeks after treatment ALP levels in the sPL group increased until week 6 and remained elevated until week 10, with the sPL/BMSCs group showing significantly higher peak ALP levels. IGF-1, TGF-β, and VEGF levels in both treatment groups were higher than in the control group. Our study demonstrated that both sPL and sPL/BMSC transplants at the bone defect site can promote bone healing and modulate bone metabolism-related factors. However, the effect of sPL/BMSCs was superior to sPL alone.