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<p>The present work aims to achieve degradation of UV treated Polypropylene (PP) by utilizing the capabilities of two bacterial strains designated as KRS102 and KRS236. Both strains were isolated from a waste dump site in Rishikesh (India), identified through 16S rDNA sequencing and compared for PP degradation ability. Detection of biosurfactant production and hydrophobicity assessment validated biodegradation potential of the isolated strains while 16S rDNA secondary structure prediction using RNAfold confirmed their thermodynamic stability. The biodegradation of PP films in terms of percent weight loss by <i>Bacillus cereus</i> KRS102 and <i>Bacillus tropicus</i> KRS236 was found to be 3.4 ± 0.20% and 2.9 ± 0.18%, respectively following 90&#xa0;days of incubation period. Strain KRS102 exhibited faster depolymerization dynamics than KRS236, as indicated by its higher biomass (protein content: 9.64 ± 3.3&#xa0;µg&#xa0;mL⁻<sup>1</sup>), greater degradation rate constant (0.000384&#xa0;day⁻<sup>1</sup>), and shorter half-life (1805&#xa0;days) as determined by kinetic modeling. EDX analysis indicated oxidative cleavage of the polymer chains. The KRS102-treated film showed a marked increase in oxygen content (27.74%) and a corresponding decrease in carbon (72.26%), reflecting extensive oxidative modification. In contrast, the control PP film exhibited minimal oxygen (0.94%) and was predominantly composed of carbon (99.10%). The FTIR study revealed changed functional group intensities and an increase in the carbonyl index. These alterations show that the biofilm matrix promotes substrate enzymatic interactions and biochemical transformations, indicating increased metabolic activity in the microbial biofilm. GC–MS and SEM analysis corroborated these findings through degradation intermediates and visible surface alterations respectively. Overall, <i>Bacillus cereus</i> KRS102 exhibited higher biodegradation efficiency than <i>Bacillus tropicus</i> KRS236, highlighting its potential as environment friendly PP degrader.</p> Graphical abstract <p></p>

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Bioprospecting of waste dump site reveals degradation potential among Bacillus cereus KRS102 and Bacillus tropicus KRS236 against UV-treated polypropylene

  • 期刊论文

  • 发表日期：

    2026年5月15日

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  • Swati Rani,
  • Himalaya Panwar,
  • Kartikey Kumar Gupta

摘要

The present work aims to achieve degradation of UV treated Polypropylene (PP) by utilizing the capabilities of two bacterial strains designated as KRS102 and KRS236. Both strains were isolated from a waste dump site in Rishikesh (India), identified through 16S rDNA sequencing and compared for PP degradation ability. Detection of biosurfactant production and hydrophobicity assessment validated biodegradation potential of the isolated strains while 16S rDNA secondary structure prediction using RNAfold confirmed their thermodynamic stability. The biodegradation of PP films in terms of percent weight loss by Bacillus cereus KRS102 and Bacillus tropicus KRS236 was found to be 3.4 ± 0.20% and 2.9 ± 0.18%, respectively following 90 days of incubation period. Strain KRS102 exhibited faster depolymerization dynamics than KRS236, as indicated by its higher biomass (protein content: 9.64 ± 3.3 µg mL⁻1), greater degradation rate constant (0.000384 day⁻1), and shorter half-life (1805 days) as determined by kinetic modeling. EDX analysis indicated oxidative cleavage of the polymer chains. The KRS102-treated film showed a marked increase in oxygen content (27.74%) and a corresponding decrease in carbon (72.26%), reflecting extensive oxidative modification. In contrast, the control PP film exhibited minimal oxygen (0.94%) and was predominantly composed of carbon (99.10%). The FTIR study revealed changed functional group intensities and an increase in the carbonyl index. These alterations show that the biofilm matrix promotes substrate enzymatic interactions and biochemical transformations, indicating increased metabolic activity in the microbial biofilm. GC–MS and SEM analysis corroborated these findings through degradation intermediates and visible surface alterations respectively. Overall, Bacillus cereus KRS102 exhibited higher biodegradation efficiency than Bacillus tropicus KRS236, highlighting its potential as environment friendly PP degrader.

Graphical abstract