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<p>Microbial enhanced oil recovery (MEOR) is an innovative method for recovering residual oil from aged oil wells but availability of indigenous microorganisms in the reservoir with high potential for MEOR and biodegradation has always been a challenge. Samples from aged oil wells were analysed using 16S rRNA ribotyping, and other microbial activity tests which include hydrocarbon degradation, sulphate reduction, nitrate reduction, acetate utilization and methane production. Hydrocarbon degradation dominated activities in all samples and sequenced data implicated the genus <i>Pseudomonas,</i> to be present in all the oil wells and dominating in OKP-7T (98%), OVH-12T (54%), SAP-12L (94%), OBN-15L (71%), ORO-6T (66%) and OKP-5S (86%). The corresponding data on anaerobic biodegradation of oil well samples when supplemented with acetate commonly found in samples showed same samples dominated by the genus <i>Pseudomonas</i> also degrading between 51 and 87% of the residual hydrocarbons in samples after 10 weeks of anaerobic incubation. Other microbial activities observed in the study were nitrate reduction where the genus <i>Tepidiphilus,</i> were found in four samples (OVH-1S, OVH-3L, OVH-12T and SAP-9S) and dominating in OVH-1S (74%). Some samples also showed methanogenic activities as evidenced by the occurrence of <i>Methanobravibacter</i> and <i>Methanothermoacter</i> in the sequenced data. Dominance of the oil wells by the genus <i>Pseudomonas</i> with high potential for biodegradation and MEOR will be of great significance to the oil industry especially when considering aged oil wells that have approached their economic limits. This study was the first to investigate the dominance of the genus <i>Pseudomonas</i> in low sulphate aged oil wells in the Niger Delta and the potential prospects of such dominance for biodegradation and MEOR applications.</p> Graphical abstract <p></p>

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Anaerobic biodegradation and activities of methanogens, acetogens, sulphate and nitrate reducing bacteria in aged oil wells from low sulphate oil producing environment

  • 期刊论文

  • 发表日期：

    2025年12月12日

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  • Chuma Okoro,
  • Effanga Ekeng,
  • Sylvester Ohwo,
  • Obinna Nwinyi

摘要

Microbial enhanced oil recovery (MEOR) is an innovative method for recovering residual oil from aged oil wells but availability of indigenous microorganisms in the reservoir with high potential for MEOR and biodegradation has always been a challenge. Samples from aged oil wells were analysed using 16S rRNA ribotyping, and other microbial activity tests which include hydrocarbon degradation, sulphate reduction, nitrate reduction, acetate utilization and methane production. Hydrocarbon degradation dominated activities in all samples and sequenced data implicated the genus Pseudomonas, to be present in all the oil wells and dominating in OKP-7T (98%), OVH-12T (54%), SAP-12L (94%), OBN-15L (71%), ORO-6T (66%) and OKP-5S (86%). The corresponding data on anaerobic biodegradation of oil well samples when supplemented with acetate commonly found in samples showed same samples dominated by the genus Pseudomonas also degrading between 51 and 87% of the residual hydrocarbons in samples after 10 weeks of anaerobic incubation. Other microbial activities observed in the study were nitrate reduction where the genus Tepidiphilus, were found in four samples (OVH-1S, OVH-3L, OVH-12T and SAP-9S) and dominating in OVH-1S (74%). Some samples also showed methanogenic activities as evidenced by the occurrence of Methanobravibacter and Methanothermoacter in the sequenced data. Dominance of the oil wells by the genus Pseudomonas with high potential for biodegradation and MEOR will be of great significance to the oil industry especially when considering aged oil wells that have approached their economic limits. This study was the first to investigate the dominance of the genus Pseudomonas in low sulphate aged oil wells in the Niger Delta and the potential prospects of such dominance for biodegradation and MEOR applications.

Graphical abstract