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<p>Orchidaceae, one of the largest family of flowering plants with approximately 30,000 accepted species, are highly sensitive to environmental changes and are considered critical indicators of ecosystem health. Therefore, effective conservation strategies are urgently required. Orchid seed storage has historically been considered challenging, but recent studies have demonstrated that it is feasible; however, pollen banking remains understudied. Pollen banking allows us to develop informed crosses, enhance genetic diversity, and aid population recovery efforts. This study presents results of isotherms and moisture stress on pollinia for some orchid species. The first results on the storability of pollinia from <i>Cattleya kautskyana</i> and <i>C. intermedia</i> under three relative humidity (RH) conditions (30%, 50%, and 100%) and three storage temperatures (5&#xa0;°C, − 18&#xa0;°C, and − 196&#xa0;°C). Pollinia were germinated before storage and after 30, 90, and 120 days. After one year, the remaining pollinia were used for pollination to assess seed capsule formation and viability. Pollinia stored at − 18&#xa0;°C under 30% and 50% RH and at − 196&#xa0;°C maintained high viability. <i>Cattleya kautskyana</i> pollinia demonstrated greater resilience, producing viable capsules and seeds across all conditions tested. In contrast, <i>C. intermedia</i> pollinia achieved 100% capsule formation when stored at − 196&#xa0;°C and 50% RH and with 97% of seed viability, but the other treatments were overall not as efficient in preserving the pollinia viability. These findings highlight the species-specific nature of orchid pollinia storage and caution against broad generalizations. Further research is needed to elucidate the relationship between pollen morphology and storage behavior to facilitate broader applications in orchid conservation.</p>

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Banking on pollen: storing germplasm to preserve genetic diversity and support ex situ conservation of two Cattleya species

  • 期刊论文

  • 发表日期：

    2025年10月10日

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  • Jessica Fontes Fileti,
  • Laís Nogueira Watanabe,
  • Ceci Castilho Custodio,
  • Jeremy Antoine Foster,
  • Nelson Barbosa Machado-Neto

摘要

Orchidaceae, one of the largest family of flowering plants with approximately 30,000 accepted species, are highly sensitive to environmental changes and are considered critical indicators of ecosystem health. Therefore, effective conservation strategies are urgently required. Orchid seed storage has historically been considered challenging, but recent studies have demonstrated that it is feasible; however, pollen banking remains understudied. Pollen banking allows us to develop informed crosses, enhance genetic diversity, and aid population recovery efforts. This study presents results of isotherms and moisture stress on pollinia for some orchid species. The first results on the storability of pollinia from Cattleya kautskyana and C. intermedia under three relative humidity (RH) conditions (30%, 50%, and 100%) and three storage temperatures (5 °C, − 18 °C, and − 196 °C). Pollinia were germinated before storage and after 30, 90, and 120 days. After one year, the remaining pollinia were used for pollination to assess seed capsule formation and viability. Pollinia stored at − 18 °C under 30% and 50% RH and at − 196 °C maintained high viability. Cattleya kautskyana pollinia demonstrated greater resilience, producing viable capsules and seeds across all conditions tested. In contrast, C. intermedia pollinia achieved 100% capsule formation when stored at − 196 °C and 50% RH and with 97% of seed viability, but the other treatments were overall not as efficient in preserving the pollinia viability. These findings highlight the species-specific nature of orchid pollinia storage and caution against broad generalizations. Further research is needed to elucidate the relationship between pollen morphology and storage behavior to facilitate broader applications in orchid conservation.