<p>Responses to biological invasions often require collaboration among actors with differing levels of capacity and varying internal constraints. The use of formal coordination mechanisms or organizational frameworks, such as the incident command system (ICS), may improve responders’ efficacy. This study employed qualitative methods to examine use of the ICS in a long-term initiative to eliminate invasive wild pigs (<i>Sus scrofa</i>) in Missouri, US. Our objectives included identifying how responders perceived the benefits, challenges, and overall performance of the ICS. There was consensus that the ongoing initiative achieved substantial progress in wild pig removals and that the ICS was essential to the initiative’s efficacy. In addition to improving interagency coordination and communication, responders reported that the ICS enhanced accountability, created unity in purpose and approach, increased overall capacity through the pooling of resources, and helped them realize efficiencies and strategic advantages through specialization of roles. Reported challenges were minimal, the primary one being initial difficulties in understanding and adjusting to certain elements of the ICS paradigm, such as reporting lines that included individuals from multiple agencies. While the ICS is typically associated with short-term (months-long) responses to emergencies, responders in Missouri continued to operate under the ICS more than five years after its adoption, reflecting both the long-term nature of their objective as well as perceived advantages associated with the ICS. This study suggests possibilities for wider ICS usage, particularly in longer-term, routine invasive species applications.</p>