Scholarlink

<p>A DNA marker is routinely used to determine the size of DNA fragments by electrophoresis in molecular biology laboratories. Thus, <i>in-house</i> produced DNA markers could be appropriate for moderately equipped laboratories. We report here a new procedure for customizing fragment sizes of DNA markers and then generating 9 fragments of DNA markers, ranging from 500 to 4600&#xa0;bp, by partial digestion of the recombinant pSY4.6-1 and pSY4.6–2 plasmids using only one restriction enzyme. DNA markers, containing 9 fragments, were obtained by digesting the recombinant plasmid, which resulted from the commercial cloning pJET1.1 and pJET1.2 plasmids and inserts of 1506&#xa0;bp or 1628&#xa0;bp, and one suitable restriction enzyme. Our <i>in-house</i> produced DNA markers, containing desirable fragment sizes, could be suitable for moderately equipped laboratories due to their simplicity, time-saving, and cost-saving procedure (approximately US$5 for materials to produce a DNA marker used for 100 agarose gel lanes) compared to the current ones widely used in most laboratories.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A flexible, cost- and time-saving method for in-house laboratory production of DNA markers

  • 期刊论文

  • 发表日期：

    2025年12月2日

查看全文
  • Uyen Quynh Nguyen,
  • Tung The Pham,
  • Loan Phuong Thi Pham,
  • Lan Thuong Thi Vo

摘要

A DNA marker is routinely used to determine the size of DNA fragments by electrophoresis in molecular biology laboratories. Thus, in-house produced DNA markers could be appropriate for moderately equipped laboratories. We report here a new procedure for customizing fragment sizes of DNA markers and then generating 9 fragments of DNA markers, ranging from 500 to 4600 bp, by partial digestion of the recombinant pSY4.6-1 and pSY4.6–2 plasmids using only one restriction enzyme. DNA markers, containing 9 fragments, were obtained by digesting the recombinant plasmid, which resulted from the commercial cloning pJET1.1 and pJET1.2 plasmids and inserts of 1506 bp or 1628 bp, and one suitable restriction enzyme. Our in-house produced DNA markers, containing desirable fragment sizes, could be suitable for moderately equipped laboratories due to their simplicity, time-saving, and cost-saving procedure (approximately US$5 for materials to produce a DNA marker used for 100 agarose gel lanes) compared to the current ones widely used in most laboratories.