Arabitol production: influence of acid and salt stress on glycerol fermentation by Komagataella pastoris, Scheffersomyces stipitis, and Pichia kluyveri in a batch system
摘要
Yeasts of the genus Pichia include osmophilic species that metabolize glycerol and produce arabitol. This research aimed to evaluate the effects of acid and salt stress on enhancing the yield of arabitol in a batch system. It also innovates in fermentation processes by proposing wild yeasts of the genus and comparing them with a yeast collection. In this study, three yeasts originally classified within the genus Pichia were evaluated: Pichia kluyveri, Pichia stipitis (Scheffersomyces stipitis), and Pichia pastoris (Komagataella phaffii). The strains were fermented individually with glycerol as a substrate. The sodium chloride concentration significantly affected the specific growth rate and the product of arabitol. The strain P. stipitis stood out for its arabitol yield (0.18 ± 0.02 g arabitol g−1 glycerol consumed) at a pH of 6.8 and a sodium chloride concentration of 0.5 M. This study contributed to the search for environmental yeasts with potential use in arabitol production. It demonstrated that environmental isolates of this genus had superior tolerance to salt stress compared with the collection strain.
Graphical Abstract